Citation: XU Jun-Li, LIU Bei-Bei, WANG Yu-Long, LI Pan, YANG Kang, WU Qin, JIANG Lan, ZHANG Hao-Ran, YANG Li-Fei, ZHANG Cun-Zheng. Lateral Flow Assay for Determination of Tebuconazole in Agricultural Products[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(11): 1823-1831. doi: 10.19756/j.issn.0253-3820.191361 shu

Lateral Flow Assay for Determination of Tebuconazole in Agricultural Products

XU Jun-Li ^1,2, ,
LIU Bei-Bei ² ,
WANG Yu-Long ² ,
LI Pan ² ,
YANG Kang ² ,
WU Qin ² ,
JIANG Lan ² ,
ZHANG Hao-Ran ² ,
YANG Li-Fei ¹ ,
ZHANG Cun-Zheng ^2,3

1.
College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China;
2.
Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;
3.
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China

Received Date: 26 June 2019
Revised Date: 15 August 2019

Fund Project: This work was supported by the National Key Research and Development Program of Chiuna (No. 2016YFD0200803-3) and the Independent Innovation Project of Jiangsu Province (No. CX (17) 1003).

A Lateral flow assay was developed for the rapid determination of Tebuconazole residues in wheat and vegetables based on the high affinity and specific anti-Tebuconazole monoclonal antibody. For the lateral flow assay development, firstly, 20 nm size colloidal gold nanoparticle was synthesized to label anti-Tebuconazole monoclonal antibody (Mab) as a detection agent. Teb-OVA (0.3 mg/mL) conjugate and goat anti-mouse IgG antibody (1 mg/mL) were coated on nitric acid fiber membrane (NC) to form the test line (T) and quality control line (C), respectively. Under the optimized conditions, the assembled immunochromatographic strip could accomplish the detection of Tebuconazole within 15 min. The Limit of detection observed by naked eyes was 6.25 ng/mL, with negligible cross-reactivity to the analogs of Tebuconazole. Meanwhile, for comparison, a high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was established to determine Tebuconazole accurately and quantitatively. The results released that these two developed methods matched each other very well. These two methods could be successfully employed for the detection of Tebuconazole in wheat, cucumber, and cabbage, high-throughput, specific and precise. Immunochromatographic strip could contribute to on-site determination, and LC-MS/MS based method provided accurate quantification.
- Tebuconazole residue,
- Immunochromatographic strip,
- High performance liquid chromatography-tandem mass spectrometry
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