Citation: FU Dong-Xu, ZHENG Ling-Na, LIU Jin-Hui, WANG Bing, ZHU Yun, WANG Meng, FENG Wei-Yue. Quantitative Analysis of Silver Nanoparticles in Single Cell by Laser Ablation Inductively Coupled Plasma-Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(9): 1390-1394. doi: 10.19756/j.issn.0253-3820.191283 shu

Quantitative Analysis of Silver Nanoparticles in Single Cell by Laser Ablation Inductively Coupled Plasma-Mass Spectrometry

FU Dong-Xu ^1,2, ,
ZHENG Ling-Na ² ,
LIU Jin-Hui ^2,3 ,
WANG Bing ² ,
ZHU Yun ¹ ,
WANG Meng ² ,
FENG Wei-Yue ²

1.
College of Physics and Materials Science, Tianjin Normal University, Tianjin 300387, China;
2.
Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China;
3.
College of Sciences, Northeastern University, Shenyang 110819, China

Received Date: 14 May 2019
Revised Date: 30 June 2019

Fund Project: This work was supported by the National Basic Research Program of China (Nos. 2016YFA0201604, 2018YFB0703504) and the National Natural Science Foundation of China (Nos. U1432241, 11505194, 11575209).

In recent years, single cell analysis has become one of hot topics in analytical chemistry. In this work, a method was developed for quantitative analysis of silver nanoparticles (AgNP) in single cells by laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS). An inkjet printer was used to produce droplets of silver standards, which were similar to the matrixes of single cells. After exposed to AgNP, 95 single cells were quantitatively analyzed by LA-ICP-MS using the droplet standards. The contents of AgNP in single cells showed a lognormal distribution, ranging from 19.1 fg to 1682 fg Ag per cell with an average of (166±188) fg Ag per cell, which was in good agreement with the average from cell population ((175±2) fg Ag per cell). LA-ICP-MS had great potential in single cell analysis and provided a new tool for studying biological effects of metal nanoparticles at a single cell level.
- Sliver nanoparticles,
- Laser ablation-inductively coupled plasma-mass spectrometry,
- Single cell analysis,
- Quantitative analysis
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