Citation: LI Shuang, HONG Meng-Rong, WANG Chun-Fang, CHEN Shu-Bing, XU Ji-Lin, CHEN Juan-Juan. Investigation of Synthetic Pathway of Photosynthetic Membrane Lipids in Nitzschiaclosterium f. Minutissima Based on ¹³C Stable-Isotope-Tracer Technique[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(6): 786-793. doi: 10.19756/j.issn.0253-3820.191257 shu

Investigation of Synthetic Pathway of Photosynthetic Membrane Lipids in Nitzschiaclosterium f. Minutissima Based on ¹³C Stable-Isotope-Tracer Technique

1.
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo 315211;
2.
Ningbo Customs Technology Center, Ningbo 315040, China

Received Date: 6 May 2019
Revised Date: 19 March 2020

Fund Project: This work was supported by the National Key R&D Program of China (No.2018YFD0901103), the K. C. Wong Magna Fund in Ningbo University, and the Projects in Zhejiang Province, China (No. LGN18B050002).

Isotope labeling in conjunction with high resolution mass spectrometry (HRMS) was used for investigation of transformation pathway in photosynthetic membrane lipids in Nitzschiaclosterium f. minutissima: diacylglycerol monogalactose (MGDG), diacylglycerol digalactose (DGDG), diacylglycerol thioglyceride (SQDG) and phosphatidylglycerol (PG), and had been demonstrated as a perfect superior tool to directly illuminate the transfer pathway of carbon in photosynthetic glycerolipids, which in contrast to previous studies where only changes in compositions were taken as evidence for these processes. The experimental results showed that 4 photosynthetic membrane lipids were labeled, and the labeling content increased gradually during the whole platform. Besides, spatial structure played a decisive role in labeling priority and provided insight into photosynthetic glycolipids synthesis in marine diatoms based on MS/MS, including suggestion of an preferably labeled of backbone-linked headgroups, and then was the glycerol skeleton and fatty acid substituents. It was also observed that ¹³C incorporated into those headgroups almost simultaneously. The method could directly and accurately illuminate the utilization of carbon atoms by photosynthetic glycerolipids in Nitzschiaclosterium f. minutissima. It provided a reference analysis platform for exploring the assimilation process of microalgae lipid biomarkers in the future by Sinonovaculaconstricta.
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沈阳化工大学材料科学与工程学院沈阳 110142

Investigation of Synthetic Pathway of Photosynthetic Membrane Lipids in Nitzschiaclosterium f. Minutissima Based on 13C Stable-Isotope-Tracer Technique

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通讯作者: 陈斌, bchen63@163.com

Investigation of Synthetic Pathway of Photosynthetic Membrane Lipids in Nitzschiaclosterium f. Minutissima Based on ¹³C Stable-Isotope-Tracer Technique