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Citation: ZOU Ting-Ting, YANG Jin-Yi, XU Zhen-Lin, WANG Hong, SUN Yuan-Ming, LEI Hong-Tao, TAN Xue-Cai, SHEN Yu-Dong. Detection of Tadalafil and Its Analogues in Health Wine and Oral Liquid by Microwell Lateral Flow Immunochromatography Assay[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(8): 1249-1257. doi: 10.19756/j.issn.0253-3820.191208 shu

Detection of Tadalafil and Its Analogues in Health Wine and Oral Liquid by Microwell Lateral Flow Immunochromatography Assay

1.
College of Food Science, South China Agricultural University, Guangzhou 510642, China;
2.
College of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning 530006, China

Received Date: 17 April 2019
Revised Date: 14 May 2019

Fund Project: This work was supported by the National Key Research and Development Program of China (No. 2018YFC1602900), the National Natural Science Foundation of China (No. 31871887), the Guangzhou Science and Technology Program (No. 201704020082), the Guangxi Science and Technology Program Key Project (No. AB18126048), the Guangdong Natural Science Foundation (No. 2018B0314005) and the Key University Scientific Research Project of Department of Education of Guangdong Province, China (No. 2018KZDXM011).

To monitor illegally added Tadalafil and its analogues in health food, polyclonal antibody was obtained by immunizing New Zealand white rabbits with a common skeleton structure hapten combined with an unsaturated linear alkyl arm hapten strategy, which could simultaneously recognize Tadalafil, Amino Tadalafil and N-Desmethyl Tadalafil. A microwell lateral flow immunochromatographic assay (mwLFIA) combined with simple sample pretreatment was developed. The LOD of mwLFIA for three Tadalafil drugs was between 0.05 and 0.30 ng/mL, the cut-off values were between 50 and 100 ng/mL, and the detection time was less than 10 min. Cross-reactivity test showed no interference from substances with similar structures, indicating a good specificity of the method against Tadalafil, Amino Tadalafil and N-Desmethyl Tadalafil. The average recoveries of three drugs in spiked health wine and oral liquid ranged from 78.7% to 117.8% with RSDs less than 15%, which had a good aggrement with the results of HPLC-MS/MS. The developed mwLFIA strip was suitable for the rapid detection of Tadalafil and its analogues in health wine and oral liquid samples.
- Tadalafils,
- Microwell lateral flow immunochromatographic assay,
- Health wine and oral liquid,
- Hapten,
- Polyclonal antibody
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