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Citation: TANG Yu-Jiao, DAI Shi-Yan, ZHOU Yu-Ting, CHENG Gui-Fang, HE Pin-Gang, FANG Yu-Zhi. A Homogeneous Electrochemical Biosensor for Detection of miRNA-21 Based on DNA-templated Click Chemistry and Catalytic Hairpin Assembly[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(7): 1029-1034. doi: 10.19756/j.issn.0253-3820.191176 shu

A Homogeneous Electrochemical Biosensor for Detection of miRNA-21 Based on DNA-templated Click Chemistry and Catalytic Hairpin Assembly

College of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, China

Received Date: 1 April 2019
Revised Date: 18 May 2019

Fund Project: This work was supported by the National Key Foundation for Exploring Scientific Instrument (No. 2011YQ150078), the Science and Technology Commission of Shanghai (No. 15142200600) and the National Natural Science Foundation of China (No. 21575024).

A homogeneous electrochemical biosensor for miRNA-21 detection based on DNA-templated click chemistry and catalytic hairpin assembly was developed. 5-Amino-2,3-dicyano-1,4-naphthoquinone (ADNQ) and 2-furanpropanoic acid (FPA) were linked to two suitable probes of hairpin structure, respectively. In the presence of miRNA-21, the catalytic hairpin assembly between the two probes could be triggered. Therefore, ADNQ and FPA linked to the probes got very close to each other and reacted by a Diels-Alder reaction to change the quinone structure of ADNQ intoto ketone, making it no longer electro-active. Thus, the current difference (ΔI) could be used to indicate the concentration of miRNA-21. The feasibility and detecting performance of the proposed biosensor were also examined by cyclic voltammetry (CV), differential pulse voltammetry (DPV) and polyacrylamide gel electrophoresis (PAGE). The biosensor was successfully applied to sensitively detect miRNA-21 in a linear concentration range from 0.1-1.0×10⁴ pmol/L with a detection limit of 0.037 pmol/L (S/N=3). The biosensor was suitable for detection of miRNA-21 in blood samples.
- DNA-templated click chemistry,
- Diels-Alder reaction,
- Catalytic hairpin assembly,
- Electrochemical biosensor
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