Citation: GAN Zhen-Fei, WANG Lu, GUO Dan, LI Da-Wei. Disposable Surface-enhanced Raman Scattering Sensor for Detection of Tyrosinase Activity[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(4): 613-619. doi: 10.19756/j.issn.0253-3820.181782 shu

Disposable Surface-enhanced Raman Scattering Sensor for Detection of Tyrosinase Activity

School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China

Received Date: 17 December 2018
Revised Date: 16 January 2019

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 21777041, 21575041) and the Shanghai Pujiang Program, China (No. 17PJD010).

A novel disposable surface-enhanced Raman scattering (SERS) sensor based on enzyme-catalyzed oxidation was developed for detecting tyrosinase (TYR) activity. The SERS sensor was fabricated by assembling the synthesized 4-thiol-catechol onto the surface of silver nanoparticles (AgNPs) which were screen printed on supporting material, and then the detection of TYR activity was accomplished according to SERS spectral changes of the SERS senor caused by the reaction between 4-thiol-catechol and TYR. The results showed that the SERS sensor had fast response to TYR (less than 5 min), and there was a linear relationship between the characteristic SERS signal and TYR concentration from 1 U/mL to 100 U/mL with limit of detection (LOD) of 0.28 U/mL (3σ). Moreover, resulting from the fingerprinting advantage of SERS technique and the specific property of the employed sensing-reaction, the developed SERS sensor had good selectivity towards TYR, which could be used for detection and evaluation of TYR and TYR inhibitor in serum.
- Surface-enhanced Raman scattering,
- Tyrosinase,
- Sensor,
- Disposable
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