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Citation: ZHOU Zhuo-Heng, LIU Ya, ZHANG Bo. Microfluidic Array Liquid Chromatography: A Proof of Principle Study[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(4): 500-507. doi: 10.19756/j.issn.0253-3820.181764 shu

Microfluidic Array Liquid Chromatography: A Proof of Principle Study

College of Chemistry & Chemical Engineering, Xiamen University, Xiamen 361005, China

Received Date: 7 December 2018
Revised Date: 20 January 2019

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 21475110, 21535007, J1310024), the Fundamental Research Funds for Central Universities of China (No. 20720160051) and the Xiamen Municipal Science and Technology Plan Project (No. 3502Z20173019).

Miniaturization of separation techniques can minimize consumption of solvent and requirement of sample size and leads to high efficiency, high speed, high resolution and high throughput analysis. In this study, a microfluidic chip-based array liquid chromatographic platform enabling multicolumn separation and detection was developed. The platform was based on the combination of glass, poly(methyl methacrylate) (PMMA) and polydimethylsiloxane (PDMS) chips and up to 8 packed chromatographic channels were manufactured on such a microfluidic device. Pressurization experiments showed that the array chip could be reliably used under pressure-driven liquid chromatographic mode. A nanoflow of 300 nL/min per column was realized through multi-stage flow splitting. We investigated feasibility and effectiveness of the 8 column array chip and obtained a high efficiency of 80000 plates per meter and a good reproducibility of RSD=1.1% for the retention time. Using a protein digest as the sample, we also explored the chip's potential applicability in high throughput separations of complex biological samples.
- Microfluidics,
- Liquid chromatography,
- Chip chromatography,
- Multicolumn array,
- High throughput
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