Citation: WANG Jiu-Ming, CHEN Jun-Hui, HE Xiu-Ping, LI Xiao-Tong, WU Dan-Ni, ZHENG Xiao-Ling, WANG Xiao-Ru. Separation and Enrichment of Domoic Acid in Seawater Using Disk-based Solid Phase Extraction[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(7): 1075-1081. doi: 10.19756/j.issn.0253-3820.181761 shu

Separation and Enrichment of Domoic Acid in Seawater Using Disk-based Solid Phase Extraction

1.
Key Laboratory for Marine Bioactive Substances and Modern Analytical Technology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China;
2.
Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266071, China;
3.
Xiamen Huaxia University, Xiamen 361024, China

Received Date: 6 December 2018
Revised Date: 14 May 2019

Fund Project: This work was supported by the Basic Scientific Fund for National Public Research Institutes of China (Nos. 2018Q08, 2016S02), the Shandong Provincial Natural Science Foundation (No. ZR2018BD027) and the NSFC-Shandong Joint Fund for Marine Ecology and Environmental Sciences (No. U1706217).

Domoic acid (DA), a type of hydrophilic toxin produced by marine diatoms, can cause memory loss, which poses a serious threat to marine culture and even human health. In this study, a new method was developed for the highly efficient accumulation and detection of trace DA in seawater by high performance liquid chromatography-ultraviolet detection/mass spectrometry (HPLC-UV/MS). In this method, a solid phase extraction disk made of sulfonated styrene-vinyl benzene copolymer was used for DA enrichment and purification from acidified seawater samples. A C₁₈ chromatographic column was employed for DA separation using acetonitrile-water solution (90%, V/V) containing 2 mmol/L ammonium acetate and 0.1% (V/V) formic acid as mobile phase. Detection wavelength was set at 242 nm. Under optimal conditions, the average recovery could still maintain at 89.3% with good precision (relative standard deviation (RSD) ≤ 4.0%) while enrichment factor could reach up to 2000. The limit of detection (S/N=3) was 2.5 ng/L. The results showed that the HPLC-UV detection developed here could meet the sensitivity requirements of DA determination in real seawater samples. This method was used to determine the seawater samples obtained from East China Sea, and it was found that the DA content in two station seawater samples was up to 2.7 μg/L. This method was efficient in determination of the concentration of DA in coastal aquaculture seawater, with easy-operation and high sensitivity.
- Domoic acid,
- Disk-based solid phase extraction,
- Seawater,
- High performance liquid chromatography
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