Citation: WANG Xiao-Fei, QIN Fei, GUO Rui, ZHANG Bo. Performance of Long Packed Nanoflow Liquid Chromatographic Columns in Proteomics Analysis[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(3): 388-393. doi: 10.19756/j.issn.0253-3820.181737 shu

Performance of Long Packed Nanoflow Liquid Chromatographic Columns in Proteomics Analysis

WANG Xiao-Fei ^1, ,
QIN Fei ² ,
GUO Rui ³ ,
ZHANG Bo ¹

1.
College of Chemistry & Chemical Engineering, Xiamen University, Xiamen 361005, China;
2.
Xiamen Medical College, Xiamen 361023, China;
3.
Column Scientific Ltd, Xiamen 361102, China

Received Date: 24 November 2018
Revised Date: 11 December 2018

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 21475110, 21535007, J1310024), the China PostDoc Foundation (No. 2018M642572), the Fujian Provincial Education and Research Fund for Young Teachers (No. JT180645), the Xiamen Municipal Science and Technology Plan Project (No. 3502Z20173019) and the Fundamental Research Funds for Central Universities of China (No. 20720160051).

Long nanoLC columns have been used in proteomics to improve the resolution and detectability of peptides to enhance protein identification number and analysis confidence. In this study, based on a nanoflow liquid chromatography-electrospray ionization mass spectrometry platform, the performance of a 1.5-m long packed nanoLC column and its applicability in proteomics were investigated. Compared with the commonly used short length nanoLC column, the long packed column achieved excellent resolution for the complex peptide mixture. The proteomics data showed that, using the 1.5-m long nanoLC column, the numbers of peptide spectrum matches, the identified peptides, the unique peptides and the protein groups were increased by 83.6%, 74.4%, 75.0% and 38.4%, respectively. The results demonstrated that the packed long nanoLC columns could be used as a new separation tool for high-resolution proteomics.
- Nanoflow liquid chromatography,
- Capillary liquid chromatography,
- Packed columns,
- Proteomics,
- Mass spectrometry
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