Citation: ZHAO Fan, SHI Guo-Yue, TIAN Yang. Simultaneous Determination of Glutamate and Calcium Ion in Rat Brain during Spreading Depression and Ischemia Processes[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(3): 347-354. doi: 10.19756/j.issn.0253-3820.181716 shu

Simultaneous Determination of Glutamate and Calcium Ion in Rat Brain during Spreading Depression and Ischemia Processes

School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, China

Received Date: 13 November 2018
Revised Date: 3 December 2018

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 21635003, 21827814) and the Innovation Program of Shanghai Municipal Education Commission (No. 201701070005E00020).

In vivo chemical analysis is an important aspect in exploring brain functions. Brain function realization depends on multiple chemical substances working together. Sole chemical detection is difficult to clarify the chemical signal transmission and disease occurrence. Thus, it is particularly important to simultaneous determination of multiple biological species in the brain. In this study, we designed and constructed a dual-functional electrochemical biosensor for simultaneous determination of glutamate and Ca²⁺. The co-modified Pt-microelectrode of GluOx and Pt NPs was served as amperometric glutamate microelectrode. By incorporating with potentiometric all-solid-state Ca-ISME, the dual function microelectrode (DFME) biosensor was developed for simultaneous monitoring glutamate and Ca²⁺ without crosstalk between the two signals. The DFME biosensor showed good selectivity towards common interferences, as well as low detection limits (0.5 μmol/L for glutamate and 1 μmol/L for Ca²⁺), which met the needs of in vivo analysis. The developed biosensor was successfully applied to real-time monitoring of glutamate and Ca²⁺ in live rat brain during spreading depression (SD) and ischemia processes. This study provided a simple and effective approach for in vivo analysis of two or more chemical substances, which was helpful for understanding of brain physiological and pathological processes.
- Glutamate,
- Calcium ion,
- Microelectrode,
- Microbiosensor,
- Spreading depression,
- Ischemia
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