Citation: GAO Jie, FANG Qun. Advances in Microfluidic Techniques for Nanoliter-Scale Protein Crystallization Screening[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(2): 169-180. doi: 10.19756/j.issn.0253-3820.181580 shu

Advances in Microfluidic Techniques for Nanoliter-Scale Protein Crystallization Screening

GAO Jie ,
FANG Qun

Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou 310058, China

Received Date: 7 September 2018
Revised Date: 24 October 2018

Fund Project: This work was supported by the Key Program and Special Project for Scientific Instrument Research of National Natural Science Foundation of China (Nos. 21435004, 21227007).

X-ray crystallography is the major approach in the determination of protein structure. To obtain high quality protein crystals that meet the diffraction requirements, researchers usually spend large amounts of reagents and samples for large-scale crystallization condition screening. Microfluidic technology can greatly reduce the consumption of protein samples in protein crystallization screening by manipulating ultramicro amounts of fluids. In this review, the recent progress in microfluidic protein crystallization screening methods based on the microbatch, vapor diffusion, free-interface diffusion and dialysis methods is introduced according to the difference in crystallization methods.
- Microfluidics,
- Protein crystallization screening,
- Nanoliter-scale,
- Review
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