Citation: YUAN Shuai, HUO Bing-Yang, ZHANG Man, WANG Yu, BAI Jia-Lei, PENG Yuan, NING Bao-An, CHEN Ping, GAO Zhi-Xian. Dual Signal Amplification Strategy for Detection of Ricin Toxin through Bio-Barcode Assay Combined with Hybridization Chain Reaction[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(1): 147-154. doi: 10.19756/j.issn.0253-3820.181504 shu

Dual Signal Amplification Strategy for Detection of Ricin Toxin through Bio-Barcode Assay Combined with Hybridization Chain Reaction

YUAN Shuai ^1,2, ,
HUO Bing-Yang ² ,
ZHANG Man ² ,
WANG Yu ² ,
BAI Jia-Lei ² ,
PENG Yuan ² ,
NING Bao-An ² ,
CHEN Ping ¹ ,
GAO Zhi-Xian ²

1.
Food Science and Engineering College, Jilin Agricultural University, Changchun 130118, China;
2.
Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Institute of Environmental and Operational Medicine, Academy of Military Medical Science, Academy of Military Science, Tianjin 300050, China

Received Date: 2 August 2018
Revised Date: 30 September 2018

Fund Project: This work was supported by the National Basic Research and Development Plan of China (No. 2017YFC1200903) and the Tianjin Science and Technology Support Key Project (No. 18YFZCNC01260).

A novel highly sensitive and specific method for detection of ricin toxin (RT) based on bio-barcode combined with hybridization chain reaction (HCR) amplification was developed. Functional gold nanoparticles (GNPs) were coated with anti-ricin polyclonal antibodies (pAb) and barcode DNA. The "mAb-RT-pAb/AuNPs/barcode DNA" sandwich structure was formed by co-identification with the anti-ricin monoclonal antibody (mAb), and HCR was further carried out by using barcode DNA as an initiation chain probe for triggering the chain-triggered biotin label, which resulted in a long double-stranded DNA with repeating units. Immediately, streptavidin labeled horseradish peroxidase (SA-HRP) was combined with biotin on long strand DNA. Finally, the chemiluminescence values were detected through adding substrates of luminol and hydrogen peroxide. The results showed that the linear range of RT was 0.61 ng/mL-5000 ng/mL, and the limit of detection (LOD) was 0.52 ng/mL(S/N=3). This method showed a wide detection range and a low detection limit. The recoveries of spiked samples in milk and water samples ranged from 83.4% to 112.4%, and the relative standard deviations (RSDs) ranged from 2.2% to 5.4%. This method exhibited great application prospects in food drinking water safety control and prevention of bioterrorism attacks.
- Ricin toxin,
- Bio-barcode,
- Hybridization chain reaction,
- Streptavidin-biotin,
- Chemiluminescence
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