Citation:
HUANG Li-Ying, FAN Dong-Jie, ZHANG Yue-Xing, MENG Chun-Ying, GU Jie. Application of Multi-element and Stable Isotope Ratios in Geographical Traceability of Trichiurus haumela Sold Online[J]. Chinese Journal of Analytical Chemistry,
;2019, 47(3): 439-446.
doi:
10.19756/j.issn.0253-3820.181422
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To explore the possibility of applying the characteristics of element composition and stable isotopes of Trichiurus haumela sold online for investigation of traceability of geographic origin, the stable isotope ratios (δ13C and δ15N) and multi-element (Na, Mg, K, Ca, Mn, Fe, Cu, Zn, As, Sr) contents of 24 batches of trichiurus haumela in different regions including Lvsi Fishing Ground, Zhoushan Fishing Ground, Qinghai Fishing Ground, and Minnan Fishing Ground were determined by liquid chromatography-isotope mass spectrometry (LC-IRMS) and inductively coupled plasma mass spectrometry (ICP-MS). Based on the differences in their stable isotope ratios and elemental composition, a discriminant model was established based on cluster analysis (CA) and principal component analysis (PCA) to trace the origin of trichiurus haumela sold online in the four regions. Analysis of variance (ANOVA) showed that the stable isotope ratios and elemental composition of trichiurus haumela sold online in each area had obvious regional characteristics, and the contents of the nine elements were significantly different. CA result indicated that the correct discrimination rate of the sample origin was 83.33%. PCA showed that the scattered points of the first three principal components (the cumulative variance contribution rate is 82.16%) were divided into four relatively concentrated areas without any overlap with each other, and could effectively distinguish the origin of trichiurus haumela sold online. In summary, the use of stable isotopes and multi-element detection combined with CA, PCA could effectively trace the origin and discriminate of trichiurus haumela sold online, had certain feasibility and application value.
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