Citation: LIU Jian-Chuan, ZHANG Hua, HUANG Hai-Chun, SHEN You-Qi, WANG Ya-Nan, ZHANG Xiao-Ping. Rapid Quantification Analysis of 3-hydroxybenzo[a]pyrene in Human Urine Using Magnetic Solid-phase Extraction Combined with Internal Extractive Electrospray Ionization Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(4): 634-639. doi: 10.19756/j.issn.0253-3820.181089 shu

Rapid Quantification Analysis of 3-hydroxybenzo[a]pyrene in Human Urine Using Magnetic Solid-phase Extraction Combined with Internal Extractive Electrospray Ionization Mass Spectrometry

1.
Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, East China University of Technology, Nanchang 330013, China;
2.
State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, Changchun 130012, China

Received Date: 2 April 2018
Revised Date: 26 December 2018

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 21605016, 21605017), the Science and Technology Planning Project at the Ministry of Science and Technology of Jiangxi Province (No. 20151BBG70038) and the Research Fund of East China University of Technology (No. DHBK2016131).

Rapid and efficient strategy for detection of polycyclic aromatic hydrocarbons (PAHs) metabolites in human urine is urgently demanded in evaluation of internal exposure of PAHs. In this study, the combination of magnetic solid-phase extraction and internal extractive electrospray ionization mass spectrometry was used to rapid and high-throughput quantification of 3-hydroxybenzopyrene (3-OHBaP) in undiluted human urine samples. Polypyrrole-coated Fe₃O₄ magnetite (termed as Fe₃O₄@Ppy) was employed as the sorbent for MSPE of urinary 3-OHBaP in undiluted human urine samples, and then the material was treated as a bulk sample, which was directly analyzed by internal extractive electrospray ionization mass spectrometry (iEESI-MS). The signal intensity of m/z 239 was linearly related with 3-OHBaP concentration over the range of 0.0005-2.50 μg/L (R²=0.9989). The limit of detection (LOD) of 3-OHBaP in undiluted urine sample was 0.1 ng/L (S/N=3). The relative standard deviations (RSDs) for six replicate measurements of 3-OHBaP concentration were below 8.0%. The concentration of 3-OHBaP in urine of smoking volunteers was significantly higher than that of non-smoking volunteers. Remarkable sensitivity of urinary 3-OHBaP detection was provided by the proposed method, showing the potential application in evaluation of trace level internal dose of PAHs exposure of human beings.
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沈阳化工大学材料科学与工程学院沈阳 110142