Citation: YAN Hui, YANG Lei, CHANG Guo-Liang, LI Xiao, NIU Mei-Ju. DNA Interaction and Cytotoxic Activity of a Chiral Amino-alcohol Schiff Base Derived Cu(II) Complex[J]. Chinese Journal of Structural Chemistry, ;2016, 35(3): 465-471. doi: 10.14102/j.cnki.0254-5861.2011-0989 shu

DNA Interaction and Cytotoxic Activity of a Chiral Amino-alcohol Schiff Base Derived Cu(II) Complex

1.
School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, Shandong 252059, China

Corresponding author: NIU Mei-Ju,
Received Date: 28 September 2015
Available Online: 12 January 2016
Fund Project: the Natural Science Foundation of China (No. 21105042) (No. ZR2013BM017)

A novel copper(II) complex based on chiral amino-alcohol derived Schiff base ligand, [Cu₄(R-L)₄(H₂O)₂]·(CH₃COOH)₂·(H₂O) (1, (R)-H₂L = (R)-3-phenyl-2-(2-hydroxy-3-methoxybenzylideneamino) propane-1-ol), was synthesized and characterized by EA, IR, UV-Vis, ESI-MS, circular dichroism spectra and single-crystal X-ray diffraction. Complex 1 crystallizes in orthorhombic, space group R2₁2₁2 with a = 15.7660(14), b = 49.526(3), c = 10.4213(9) Å, V = 8137.2(12) Å³, Z = 4, C₇₂H₈₁Cu₄N₄O₁₉, M_r = 1560.57, m = 1.096 mm^-1, F(000) = 3244, Flack = 0.06(3), the final R = 0.0924 and wR= 0.2451 (I > 2s(I)) for 41108 observed reflections. The interactions of the complex with calf thymus DNA (CT-DNA) were investigated by some spectroscopic technique methods. The results show the complex exhibits strong binding with CT-DNA. In addition, in vitro cytotoxicity test of 1 towards four kinds of human cancerous cell lines (HeLa, HL-60, Caco-2 and A549) showed substantial cytotoxic activity. The experimental investigations indicated that the chirality of complex 1 play an important role in cytotoxicity and interactions with DNA.
- chiral Schiff base,
- in vitro cytotoxicity,
- DNA-binding
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