Citation: Zhiran Duan, Haijing Qu, Xiangdong Xue. Construction and characterization of supramolecular assembly-based nanodrug delivery systems: a teaching practice in supramolecular chemistry experiment[J]. University Chemistry, ;2026, 41(7): 239-249. doi: 10.12461/PKU.DXHX202505021 shu

Construction and characterization of supramolecular assembly-based nanodrug delivery systems: a teaching practice in supramolecular chemistry experiment

School of Pharmaceutical Science, Shanghai Jiao Tong University, Shanghai 200240, China

Corresponding author: Xiangdong Xue, xuexd@sjtu.edu.cn
Received Date: 8 May 2025
Revised Date: 27 August 2025

Guided by the strategic initiative to advance “New Medical Science” education and cultivate high-quality interdisciplinary talents, this study developed an integrated teaching experiment that bridges cutting-edge drug delivery research with practical nanomedicine applications. The experiment synergistically combines theoretical knowledge of supramolecular chemistry with hands-on practice, employing chlorin e6 and temozolomide as model drugs to prepare a nano-drug delivery system through ultrasound-assisted supramolecular assembly. We systematically examined how varying drug ratios affect the system’s performance. Successful supramolecular assembly was verified by aggregation-induced fluorescence quenching, while dynamic light scattering (DLS) characterized the system’s hydrodynamic size and zeta potential. Drug encapsulation efficiency was quantified using ultraviolet spectrophotometry. This innovative approach overcomes limitations of conventional nano-drug delivery systems, such as low drug-loading capacity and excipient-related safety concerns, by producing pure drug nanoparticles through excipient-free supramolecular assembly. With its straightforward protocol, well-defined scientific principles, and excellent scalability, this experiment effectively equips students with core competencies in nanodrug delivery system development while enhancing their conceptual understanding. It serves as an exemplary model for training interdisciplinary “New Medical Science” professionals who thoroughly comprehend fundamental principles, demonstrate innovation capabilities, and value practical application.
- Supramolecular assembly,
- Nanoparticles,
- Drug delivery,
- Experimental teaching
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