Citation: KOU Wei, ZHANG Hua, LU Hai-Yan, ZHU Li-Xue, LIU Ai-Ying, HUANG Ke-Ke, CHEN Huan-Wen. Determination of Phospholipids in Soybean by Titanium Dioxide Nanowires Array Enrichment Combined with Internal Extractive Electrospray Ionization Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(12): 1913-1922. doi: 10.11895/j.issn.0253-3820.181481 shu

Determination of Phospholipids in Soybean by Titanium Dioxide Nanowires Array Enrichment Combined with Internal Extractive Electrospray Ionization Mass Spectrometry

KOU Wei ^1, ,
ZHANG Hua ¹ ,
LU Hai-Yan ¹ ,
ZHU Li-Xue ¹ ,
LIU Ai-Ying ¹ ,
HUANG Ke-Ke ¹ ,
CHEN Huan-Wen ^1,2

1.
State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, Jilin University, Changchun 130012, China;
2.
Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, East China Institute of Technology, Nanchang 330013, China

Received Date: 23 July 2018
Revised Date: 28 September 2018

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 21427802, 21605016, 21565004) and the Science and Technology Planning Project at the Ministry of Science and Technology of Jiangxi Province, China (No. 20171ACG70015).

Titanium dioxide (TiO₂) nanowires array prepared on the fluorine-doped tin oxide (FTO) substrate by the hydrothermal synthesis method was combined with internal extractive electrospray ionization mass spectrometry (iEESI-MS), allowing selective enrichment and direct analysis of the phospholipids from soybean. The experimental parameters including ratio of trifluoroacetic acid (TFA) in methanol, adsorption time, composition, and flow rate of eluent were investigated. The results showed that the optimal signal of phospholipids was obtained under the optimized conditions such as the extraction solution of 2% (V/V) TFA in methanol, adsorption time of 60 s, eluent of 1.5% (V/V) ammonia in methanol and flow rate of 7 μL/min. A total of 18 kinds of phospholipids were detected from the grinding soybean solution. Based on these components, the soybeans from Heilongjiang, Jiangsu, Sichuan, Shandong and Henan were preliminarily separated by principal component analysis (PCA). The limits of detection (S/N ≥ 3) were 0.01 μg/L and the relative standard deviations (RSDs, n=3) were 1.6%-6.5%, showing high sensitivity in the quantitative analysis of phospholipids from soybean. Besides, accurate and rapid screening of the quality of commercial soybean oil was also achieved. This method was featured by less sample consumption, rapid analysis and simple operation, and could be used for direct and rapid detection of phospholipids in soybean.
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