Citation: WANG Zhen-Zhao, WEI Bin-Bin, CHEN Zheng, ZHU Hong-Wei, SHEN Gui-Ping, FENG Jiang-Hua. ¹H Nuclear Magnetic Resonance-based Investigation of Uremia by Metabolomic Analysis[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(9): 1415-1423. doi: 10.11895/j.issn.0253-3820.181286 shu

¹H Nuclear Magnetic Resonance-based Investigation of Uremia by Metabolomic Analysis

1.
Department of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, Xiamen University, Xiamen 361005, China;
2.
The First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, China

Received Date: 30 April 2018
Revised Date: 15 July 2018

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 31671920, 81272581).

At present, little is known about metabolic mechanism of uremia. In this study, uremia was investigated by combination of NMR spectroscopy, statistical analysis and urinary biochemical assays to provide the basis of the metabolic characteristics of uremia. The characteristic metabolites of uremia were identified from the urine of the uremia patients. The results showed that the urinary levels of 2-hydroxyisobutyrate, 3-hydroxybutyrate, acetone, butyrate, glutamate, sarcosine, creatinine, lysine, N,N-dimethylglycine, citrate, asparagine, ethanol and ethanolamine were reduced, while the urinary levels of branched-chain amino acids (including leucine, valine and isoleucine), taurine, lactate and glucose were higher in uremia patients than that in the healthy subjects. The corresponding disturbed metabolic pathways were derived from the characteristic metabolites, in which amino acid, energy and lipid metabolisms were involved. The specific characteristic metabolites will help to understand the underlying biochemical mechanisms of the onset of uremia.
- Nuclear magnetic resonance,
- Metabonomics,
- Uremia,
- Statistical analysis,
- Metabolic pathway analysis
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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

1H Nuclear Magnetic Resonance-based Investigation of Uremia by Metabolomic Analysis

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通讯作者: 陈斌, bchen63@163.com

¹H Nuclear Magnetic Resonance-based Investigation of Uremia by Metabolomic Analysis