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Citation: SUN Jing-Jing, SONG Qi, MU Ying. Microfluidic Chip for Methamphetamine Detection Based on Time-resolved Fluoroimmunoassay[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(7): 1116-1121. doi: 10.11895/j.issn.0253-3820.181216 shu

Microfluidic Chip for Methamphetamine Detection Based on Time-resolved Fluoroimmunoassay

Zhejiang University, Institute of Cyber-System and Control, State Key Laboratory of Industrial Control Technology, Research Center for Analytical Instrumentation, Hangzhou 310027, China

Received Date: 31 March 2018
Revised Date: 2 May 2018

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 81741145) and the Open Research Project of the State Key Laboratory of Industrial Control Technology, Zhejiang University, China (No. ICT1800381).

A novel polydimethylsiloxane (PDMS)-poly-L-lysine (PLL) microfluidic chip, comprised of 24 reaction channels with 2.5 μL volume of each reaction channel only, was proposed for quantitative analysis of methamphetamine (MET) based on time-resolved immunoassay techniques. The chip utilized the adsorption characteristics of PDMS and PLL towards proteins to immobilize MET complete antigens (MET-BSA) on the surface of reaction channel. Then the competition reaction could happen between MET-Ag in the sample solution and MET-BSA on the inner surfaces of the reaction channels with MET-Ab in the reagent. The surface of latex microspheres was labeled by lanthanide, which could emit red fluorescence under the exposure of ultraviolet (UV). Based on the principle of competitive immunoassay, the more MET-Ag, the less the fluorescence intensity in the reaction channel. The detection results of this chip were acquired using UV-irradiation fluorescence imaging method. With this method, 24 samples could be detected and analyzed simultaneously on a chip by just taking the fluorescence image of the chip. The method allows the detection of MET antigens ranging from 100 ng/mL to 3000 ng/mL, with less sample consumption and high-throughput. This chip is suitable for the police preliminary screening work and has a good application prospects.
- Time-resolved fluoroimmunoassay,
- Methamphetamine,
- Competitive immunoassay,
- Microfluidic chip
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2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
15. [15]
16. [16]
17. [17]
18. [18]
19. [19]
20. [20]
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