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Citation: XU Jie, LIN Bin, WANG Ya, XU Zhi-Ai, CHENG Gui-Ying, ZHANG Wen. Label-free Fluorescent DNA Detection Strategy Based on Hybridization Chain Reaction and Vacant Site-Binding Molecule[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(7): 1095-1101. doi: 10.11895/j.issn.0253-3820.181142 shu

Label-free Fluorescent DNA Detection Strategy Based on Hybridization Chain Reaction and Vacant Site-Binding Molecule

XU Jie ^1, ,
LIN Bin ¹ ,
WANG Ya ¹ ,
XU Zhi-Ai ¹ ,
CHENG Gui-Ying ² ,
ZHANG Wen ¹

1.
School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, China;
2.
College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, China

Received Date: 6 March 2018
Revised Date: 25 March 2018

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 21775046, 21675055).

A label-free and enzyme-free fluorescent DNA sensor based on hybridization chain reaction (HCR) and vacant site-binding molecule was developed. By rationally incorporating a cytosine in hairpin DNAs for HCR and utilizing vacant site-binding fluorophore ATMND, sensitive DNA detection was achieved, resulting from the large amount of vacant sites presented on the long DNA nanostructures upon HCR. Gel electrophoresis and atomic force microscopy were performed to characterize the hybridization chain reaction. Under the optimized conditions, the method could sensitively detect the target DNA with the low detection limit of 2.0 nmol/L. The method shows great potential in signal amplification for DNA detection with advantages such as simple, label-free and low-cost.
- Hybridization chain reaction,
- Vacant site,
- Fluorescence,
- Signal amplification,
- Label-free
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