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Citation: YE Si-Jian, GONG Xiao-Yun, LI Ming, LIU Da-Jun, JIANG You, FANG Xiang. Research on Peptide Ion Charge Control Method Based on Proton Transfer Ion/Ion Reaction with a Dual-Polarity Ion Trap Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(7): 1084-1088. doi: 10.11895/j.issn.0253-3820.171422 shu

Research on Peptide Ion Charge Control Method Based on Proton Transfer Ion/Ion Reaction with a Dual-Polarity Ion Trap Mass Spectrometry

1.
Changchun University of Science and Technology, Department of Chemistry & Environmental Engineering, Changchun 130022, China;
2.
National Institute of Metrology, Beijing 100029, China

Received Date: 13 November 2017
Revised Date: 10 May 2018

Fund Project: This work was supported by the National Key Research and Development Program of China (No. 2016YFF0200504) and the National Natural Science Foundation of China (No. 21605135).

Electrospray ionization is most commonly used in mass spectrometry for analysis of biological molecules such as peptides and proteins. However, peptides and proteins ions produced by electrospray ionization usually have multiple charges, and produce multiple spectrum peaks, making the mass spectrum complicated. Gas phase proton transfer ion/ion reaction can effectively regulate the charge states of peptides and proteins ions after electrospray ionization, simplifying the spectrogram, and thus is significant to the analysis of complex proteins and peptides samples. In this study, a dual-polarity linear ion trap (LIT) mass spectrometer was used to control the charge state of peptide ions by proton transfer ion/ion reaction. The detection effect of the method was verified by using glutathione (oxidation type), oxytocin and dynorphin as typical samples. The results showed that this method could remove excess charges in the positive ions. When injecting enough negative ions for eaction, peptide ions with multiple charge valence state could be reduced to the minimum, therefore simplify the spectrogram effectively.
- Proton transfer reaction,
- Peptide,
- Mass spectrometry,
- Positive and negative ion/ion reaction
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