Citation: LYU Piao-Piao, XIE Dan-Dan, ZHANG Zhao-Hui. Fluorescence Carbon Dot-based Imprinted Polymer for Highly Selective Detection of Bovine Hemoglobin[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(6): 917-924. doi: 10.11895/j.issn.0253-3820.171417 shu

Fluorescence Carbon Dot-based Imprinted Polymer for Highly Selective Detection of Bovine Hemoglobin

1.
College of Chemistry and Chemical Engineering, Jishou University, Jishou 416000, China;
2.
National Demonstration Center for Experimental Chemistry Education, Jishou University, Jishou 416000, China;
3.
State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, China

Received Date: 13 November 2017
Revised Date: 30 March 2018

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 21767011, 21565014) and the Innovation Fund Designated for Graduate Students of Jishou University (Nos. JDY16016, JGY201756).

A novel fluorescent imprinted polymer (CDs@MIP) with selective recognition of hemoglobin was prepared by the sol-gel method using fluorescent carbon dots as the carrier material, 3-aminopropyltrieth-oxysilane as the functional monomer, tetraethoxysilane as the crosslinking agent and bovine hemoglobin as template molecule. The results of IR and scanning electron microscopy showed that the molecularly imprinted polymer was coated on the surface of fluorescent carbon dots. The CDs@MIP showed selective recognition properties for bovine hemoglobin with an imprinting factor of 4.60. Also the adsorption ability and specific recognition performance of CDs@MIP were investigated, and it was found that the CDs@MIP had high selectivity toward bovine hemoglobin, and the selection factors for ovalbumin, bovine serum albumin and human serum albumin were 4.38, 4.73 and 3.66, respectively. Under the optimal conditions, the linear range of CDs@MIP for bovine hemoglobin was 0.1-10.0 μmol/L and the detection limit was 23.0 nmol/L. The CDs@MIP was successfully used for the determination of bovine hemoglobin in bovine blood samples with recoveries of 99.0%-102.5%.
- Molecular imprinting,
- Bovine hemoglobin,
- Fluorescent carbon dots
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沈阳化工大学材料科学与工程学院沈阳 110142