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Citation: LU Jin, REN Xue, LIU Xiang-Liang, LIU Ze-Feng. A Metabonomics Study of Cervical Cancer by Ultra Performance Liquid Chromatography Coupled with Quadrupole Orbitrap Tandem Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(1): 67-73. doi: 10.11895/j.issn.0253-3820.171412 shu

A Metabonomics Study of Cervical Cancer by Ultra Performance Liquid Chromatography Coupled with Quadrupole Orbitrap Tandem Mass Spectrometry

1.
Cancer Center, The First Hospital of Jilin University, Changchun 130021, China;2 Department of Gynecologic Oncology, The First Hospital of Jilin University, Changchun 1300121, China;3 Department of General Surgery, The Second Hospital of Jilin University, Changchun 130041, China

Received Date: 9 November 2017
Revised Date: 5 December 2017

This work aims to analyze and compare the potential biomarkers between cervical cancer (CC) patients and healthy controls (HC). The urine samples of 11 CC patients (age (45.65 ±5.6) years) and 11 HC patients (age (45.9 ±3.2) years) were collected. The metabolites of urine samples from CC and HC were analyzed by using ultra performance liquid chromatography coupled with quadrupole orbitrap tandem mass spectrometry (UHPLC-Q-Orbitrap MS/MS), which could provide evidence for early diagnose and disease pathway. The LC-MS data of urines were analyzed by principal components analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) to identify the potential biomarkers. Urine samples of CC patients were successfully discirminated from those of healthy controls. A total of twelve significant metabolites were found and identified as potential biomarkers according to the established UHPLC-Q-Orbitrap MS and MS/MS method. Identification of biomarkers between CC and HC patients may play an important role in the study of mechanism of UC and its pathway.
- Cervical cancer,
- Ultra performance liquid chromatography coupled with quadrupole orbitrap tandem mass spectrometry,
- Metabonomics,
- Urine,
- Biomarkers
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