Citation: DU Zai-Hui, LI Xiang-Yang, TIAN Jing-Jing, TIAN Hong-Tao, XU Wen-Tao. Establishment and Application of Functional Nucleic Acid Biosensor for Detection of Mercury Ion[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(6): 947-951. doi: 10.11895/j.issn.0253-3820.171390 shu

Establishment and Application of Functional Nucleic Acid Biosensor for Detection of Mercury Ion

1.
College of Food Science and Technology, Agricultural University of Hebei, Baoding 071000, China;
2.
Beijing Advanced Innovation Center for Food Nutrition and Human Health, China Agricultural University, Beijing 100000, China;
3.
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100000, China;
4.
Food Science and Engineering College, Beijing University of Agriculture, Beijing 102206, China

Received Date: 5 November 2017
Revised Date: 8 March 2018

Fund Project: This work was supported by the Beijing New-StarPlan of Science and Technology (No. xxjc201721), the National Natural Science Foundation of China (No.31671922) and Hebei Province Food Science and Engineering First-Rate Discipline Construction Projects (No.1090004).

Excessive mercury ions have negative effects on individual health. So, it is of great significance to develop a method for rapid, sensitive and specific detection of Hg²⁺. In this study, the method for detection of Hg²⁺ was developed based on specific T-Hg-T mismatche and G-quadruplex. The template sequence mainly included the recognition region which could combine with Hg²⁺ specifically, the rich G region which could form G-quadruplex, and the speacer 18 partition zone. The target sequence mainly included 5' ends combining area and 3' end T-rich region. Templates and targets could be combined and the elongation was triggered only in the presence of Hg²⁺. Furthermore, the G-rich sequences were stripped off the templates and could form a single-stranded structure, because Spacer 18 had partition function. The G-quadruplex was formed with the K⁺ and hemin, and catalyzed H₂O₂-2,2-azinobis (3-ethylbenzothiozoline)-6-sulfonic acid(ABTS) reaction with color variations. The detection could be done within 35 min, and the Hg²⁺ concentration exhibited a linear correlation with absorbance at 414 nm within range of 20-500 nmol/L, with a detection limit of 16.5 nmol/L (3σ). The recoveries of Hg²⁺ spiked in tap water were 98.5%-103.5%. The method exhibited the advantages such as simple operation, low cost and short time, and operation value in emergency treatment and real-time environmental detection.
- Mercury ion,
- G-quadruplex,
- Spacer arm,
- Functional nucleic acid,
- Biosensor
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1.
沈阳化工大学材料科学与工程学院沈阳 110142