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Citation: HUANG Hua-Bin, FU Qi, HU Jia, YOU Qi-Hua, ZHUANG Zhi-Xia, YANG Chao-Yong, WANG Xiao-Ru. Magnetic Microsphere with Surface Charge Switching-based Microfluidic Chip for DNA Extraction[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(9): 1372-1378. doi: 10.11895/j.issn.0253-3820.171318 shu

Magnetic Microsphere with Surface Charge Switching-based Microfluidic Chip for DNA Extraction

HUANG Hua-Bin ^1, ,
FU Qi ¹ ,
HU Jia ¹ ,
YOU Qi-Hua ¹ ,
ZHUANG Zhi-Xia ^1,2 ,
YANG Chao-Yong ² ,
WANG Xiao-Ru ^1,2

1.
XiaMen HuaXia College, Department of Science and Technology for Inspection, Xiamen 361024, China;
2.
College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China

Received Date: 10 October 2017
Revised Date: 8 July 2018

Fund Project: This work was supported by the National Spark Program (No. 2015GA721002), the Open Foundation from State Key Laboratiory of Loess and Quaternary Geology(No. SKLLQG1607) and the Project of Science and Technology for Young Teachers in Fujian Province, China(No. JAT170817)

Self-made surface charge switching amino-SiO₂@Fe₃O₄ magnetic composite microspheres were applied to microfluidic chip, and DNA solid phase extraction chip of polymethyl methacrylate (PMMA) was fabricated by laser etching and hot pressing bonding. The magnetic beads that could be controlled by permanent magnet were injected into the chip channel to obtain a magnetic bead chip for genomic DNA extraction in human whole blood. Under the optimized extraction conditions (with 80 μg beads, flow rate for adsorption, elution and desorption were 10, 5 and 20 μL/min, respectively), the extracted products were subjected to gel electrophoresis and PCR test. The results showed that the genomic DNA with high purity was extracted from the whole blood by magnetic bead microfluidic chip. The extraction efficiency was about 35%. The gel electrophoresis band of the extract was consistent with the genomic DNA extracted by commercial reagent kit. The extract solution could be used for further PCR reaction.
- Surface charge switching,
- Magnetic beads,
- Microfluidic chip,
- DNA extraction
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