Citation: WANG Hong-Ya, YIN Bin-Cheng, YE Bang-Ce. Specific microRNA Detection Based on Surface Plasmon-Enhanced Energy Transfer Between Gold Nanoparticles and Silver Nanoclusters[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(12): 2018-2025. doi: 10.11895/j.issn.0253-3820.171317 shu

Specific microRNA Detection Based on Surface Plasmon-Enhanced Energy Transfer Between Gold Nanoparticles and Silver Nanoclusters

1.
School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832000, China
2.
Lab of Biosystem and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, Shanghai 200237, China

Corresponding author: YE Bang-Ce, bcye@ecust.edu.cn
Received Date: 11 October 2017
Accepted Date: 1 November 2017

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 21675052, 21335003, 21575089) and the Fundamental Research Funds for the Central Universities, Chinathe National Natural Science Foundation of China 21335003the National Natural Science Foundation of China 21675052the National Natural Science Foundation of China 21575089

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There is high demand for a sensitive method for miRNA detection in clinical diagnosis. In this work, we developed a method for miRNA detection based on the surface plasmon-enhanced energy transfer (SPEET) between gold nanoparticles (AuNPs) and silver nanoclusters (AgNCs), coupled with DNA polymerase and nicking enzyme-assisted isothermal amplification for target recycling. Two DNA probes (Probe a and Probe b) were assembled onto the surface of AuNPs to form Probe b-Probe a-AuNP conjugates. Probe a consisted three domains: the complementary sequence of miRNA, the specific site of the nicking enzyme, and the self-assembly sequence for AgNCs. The 3' end of Probe a was modified with thiol as a binding site for AuNPs. The SPEET of AgNCs and AuNPs was inhibited when miRNA was added to produce the dumbbell shaped template by polymerase. The template could promote synthesis of AgNCs, resulting in replacement and subsequently recycling of the target molecule for signal amplification. In comparison with the traditional method of miRNA detection with commercial RT-PCR kits, this method avoided the process of reverse transcription and was easy to perform. In addition, this method with a detection limit of 2.5×10^-11 mol/L was cost-effective, label-free, and highly selective for detecting miRNA, and could be applied to the analysis of miRNA in biological samples.
- MicroRNA detection,
- Gold nanoparticles,
- Silver nanoclusters,
- Surface plasmon-enhanced energy transfer
1. [1]
  Rana T M. Nat. Rev. Mol. Cell Biol., 2007, 8(1):23-36 doi: 10.1038/nrm2085
2. [2]
  Lee R C, Feinbaum R L, Ambros V. Cell, 1993, 75(5):843-854 doi: 10.1016/0092-8674(93)90529-Y
3. [3]
  Griffiths-Jones S, Saini H K, Dongen S V, Enright A J. Nucleic Acids Res., 2008, 36(SI):D154-D158
4. [4]
  Brennecke J, Hipfner D R, Stark A, Russell R B, Cohen S M. Cell, 2003, 113(1):25-36 doi: 10.1016/S0092-8674(03)00231-9
5. [5]
  Wang Y, Keys D N, Auyoung J K, Chen C. J. Cell Physiol., 2009, 218(2):251-255 doi: 10.1002/jcp.v218:2
6. [6]
  Dostie J, Mourelatos Z, Yang M, Sharma A, Dreyfuss G. RNA, 2003, 9(2):180-186 doi: 10.1261/rna.2141503
7. [7]
  Xu P, Vernooy S Y, Guo M, Hay B A. Curr. Biol., 2003, 13(9):790-795 doi: 10.1016/S0960-9822(03)00250-1
8. [8]
  Chen C Z, Li L, Lodish H F, Bartel D P. Science, 2004, 303(5654):83-86 doi: 10.1126/science.1091903
9. [9]
  Calin G A, Dumitru C D, Shimizu M, Bichi R, Zupo S, Noch E, Aldler H, Rattan S, Keating M, Rai K. Proc. Natl. Acad. Sci. USA, 2002, 99(24):15524-15529 doi: 10.1073/pnas.242606799
10. [10]
  Michael M Z, O' Connor S M, van Holst Pellekaan N G, Young G P, James R J. Mol. Cancer Res., 2003, 1(12):882-891
11. [11]
  Metzler M, Wilda M, Busch K, Viehmann S, Borkhardt A. Genes Chromosomes Cancer, 2004, 39(2):167-169 doi: 10.1002/(ISSN)1098-2264
12. [12]
  Takamizawa J, Konishi H, Yanagisawa K, Tomida S, Osada H, Endoh H, Harano T, Yatabe Y, Nagino M, Nimura Y. Cancer Res., 2004, 64(11):3753-3756 doi: 10.1158/0008-5472.CAN-04-0637
13. [13]
  Eis P S, Tam W, Sun L, Chadburn A, Li Z, Gomez M F, Lund E, Dahlberg J E. Proc. Natl. Acad. Sci.USA, 2005, 102(10):3627-3632 doi: 10.1073/pnas.0500613102
14. [14]
  Chan J A, Krichevsky A M, Kosik K S. Cancer Res., 2005, 65(14):6029-6033 doi: 10.1158/0008-5472.CAN-05-0137
15. [15]
  Kluiver J, Poppema S, De J D, Blokzijl T, Harms G, Jacobs S, Kroesen B J, Van den Berg A. J. Pathol., 2005, 207(2):243-249 doi: 10.1002/path.v207:2
16. [16]
  Mitchell P S, Parkin R K, Kroh E M, Fritz B R, Wyman S K, Pogosova-Agadjanyan E L, Peterson A, Noteboom J, O'Briant K C, Allen A. Proc. Natl. Acad. Sci. USA, 2008, 105(30):10513-10518 doi: 10.1073/pnas.0804549105
17. [17]
  Válóczi A, Hornyik C, Varga N, Burgyán J, Kauppinen S, Havelda Z. Nucleic Acids Res., 2004, 32(22):e175 doi: 10.1093/nar/gnh171
18. [18]
  Pena J T G, Cherin S L, Rouhanifard S H, Janos L, Markus H, Aleksandra M, Cindy L, Daniel H, Philipp B, Mihaela Z. Nat. Meth., 2009, 6(2):139-141 doi: 10.1038/nmeth.1294
19. [19]
  Raymond C K, Roberts B S, Garrettengele P, Lim L P, Johnson J M. RNA, 2005, 11(11):1737-1744 doi: 10.1261/rna.2148705
20. [20]
  Chen C, Ridzon D A, Broomer A J, Zhou Z, Lee D H, Nguyen J T, Barbisin M, Xu N L, Mahuvakar V R, Andersen M R. Nucleic Acids Res., 2005, 33(20):e179 doi: 10.1093/nar/gni178
21. [21]
  Jonstrup S P, Koch J, Kjems J. RNA, 2006, 12(9):1747-1752 doi: 10.1261/rna.110706
22. [22]
  Chen A, Ma S, Zhuo Y, Chai Y, Yuan R. Anal. Chem., 2016, 88(6):3203-3210 doi: 10.1021/acs.analchem.5b04578
23. [23]
  Jia H, Li Z, Liu C, Cheng Y. Angew. Chem. Int. Ed., 2010, 49(32):5498-5501 doi: 10.1002/anie.201001375
24. [24]
  Yin B C, Liu Y Q, Ye B C. J. Am. Chem. Soc., 2012, 134(11):5064-5067 doi: 10.1021/ja300721s
25. [25]
  Chen C W, Wang C H, Wei C M, Hsieh C Y, Chen Y T, Chen Y F, Lai C W, Liu C L, Hsieh C C, Chou P T. J. Phys. Chem. C, 2009, 114:799-802
26. [26]
  Liu J M, Chen J T, Yan X P. Anal. Chem., 2013, 85:3238-3245 doi: 10.1021/ac303603f
27. [27]
  Vetten M A, Tlotleng N, Tanner Rascher D, Skepu A, Keter F K, Boodhia K, Koekemoer L A, Andraos C, Tshikhudo R, Gulumian M. Part. Fibre Toxicol., 2013, 10:50 doi: 10.1186/1743-8977-10-50
28. [28]
  Osborn L, Kunkel S, Nabel G J. Proc. Natl. Acad. Sci. USA, 1989, 86(7):2336-2340 doi: 10.1073/pnas.86.7.2336
29. [29]
  Bi S, M. Chen X, Jia Q, Dong Y. Nanoscale, 2015, 7:3745-3753 doi: 10.1039/C4NR06603K
30. [30]
  Zhao J J, Jin X, Vdovenko M, Zhang L L, Sakharov I Y, Zhao S L. Chem. Commun., 2015, 51:11092-11095 doi: 10.1039/C5CC04381F
31. [31]
  Wang J, Yin B C, Ye B C. Biosens. Bioelectron., 2016, 80:366-372 doi: 10.1016/j.bios.2016.02.005
32. [32]
  Wang J, Li R D, Yin B C, Ye B C. Analyst, 2015, 140:6306-6312 doi: 10.1039/C5AN01350J
33. [33]
  Degliangeli F, Kshirsagar P, Brunetti V, Pompa P P, Fiammengo R. J. Am. Chem. Soc., 2014, 136 (6):2264-2267 doi: 10.1021/ja412152x
34. [34]
  Xi Q, Zhou D M, Kan Y Y, Ge J, Wu Z K, Yu R Q, Jiang J H. Anal. Chem., 2014, 86:1361-1365 doi: 10.1021/ac403944c
35. [35]
  Li R D, Yin B C, Ye B C. Biosens. Bioelectron., 2016, 86:1011-1016 doi: 10.1016/j.bios.2016.07.042
36. [36]
  Li R D, Wang J, Yin B C, Ye B C. Biosens. Bioelectron., 2015, 77:995-1000
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