Citation: WEI Tian-Tian, LIANG Ru-Ping, QIU Jian-Ding. Thrombin Biosensor Based on Rare Earth Ion Mediated Fluorescence Switch of Graphene Quantum Dots[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(12): 1915-1920. doi: 10.11895/j.issn.0253-3820.171293 shu

Thrombin Biosensor Based on Rare Earth Ion Mediated Fluorescence Switch of Graphene Quantum Dots

College of Chemistry, Nanchang University, Nanchang 330031, China

Corresponding author: QIU Jian-Ding, jdqiu@ncu.edu.cn
Received Date: 5 October 2017
Accepted Date: 7 November 2017

Fund Project: the National Natural Science Foundation of China 21675078the Program for Major Academic and Technical Leaders of Jiangxi Province 20162BCB22013This work was supported by the National Natural Science Foundation of China (Nos. 21475056, 21675078), and the Program for Major Academic and Technical Leaders of Jiangxi Province (Nos. 20123BCB22003, 20162BCB22013)the Program for Major Academic and Technical Leaders of Jiangxi Province 20123BCB22003the National Natural Science Foundation of China 21475056

Figures(6)

A label-free method for sensitive and selective detection of thrombin (Tb) was constructed based on rare earth ion mediated fluorescence switch of graphene quantum dots (GQDs). Rare earth ion (Er³⁺) can assemble onto the surface of GQDs through the coordination interaction between Er³⁺ ions and the carboxylate groups located on the surface or edge of the GQDs, resulting in the aggregation of the GQDs and thereby decrease of the fluorescence of the GQDs. In the presence of Tb, the oxygen and nitrogen-donor atoms in Tb can coordinate with Er³⁺ ions and compete with the carboxylate groups on GQDs to coordinate Er³⁺ ions, thus the interaction between GQDs and Er³⁺ ions is reduced, which lead to the restoration of the GQDs fluorescence. In this study, the sensing mechanism was demonstrated by transmission electron microscopy, atomic force microscope, Fourier transform infrared spectroscopy, UV-Vis absorption and fluorescence spectroscopy. The limit of detection for Tb assay was as low as 0.049 nmol/L. Moreover, this assay was successfully applied to the selective determination of Tb in real samples.
- Rare earth ion,
- Graphene quantum dots,
- Fluorescence switch,
- Thrombin,
- Sensing
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