Citation: HE Xin-Yu, WANG Bing, ZHOU Yang-Yang, BIAN Xiao-Jun, YAN Juan. Enrichment and Identification of Metallothionein by Functionalized Nano-Magnetic Particles and Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(7): 1069-1076. doi: 10.11895/j.issn.0253-3820.171285 shu

Enrichment and Identification of Metallothionein by Functionalized Nano-Magnetic Particles and Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry

HE Xin-Yu ^1, ,
WANG Bing ¹ ,
ZHOU Yang-Yang ¹ ,
BIAN Xiao-Jun ^1,2,3 ,
YAN Juan ^1,2,3

1.
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China;
2.
Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai 201306, China;
3.
Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation(Shanghai), Ministry of Agriculture, Shanghai 201306, China

Received Date: 1 October 2017
Revised Date: 27 February 2018

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 21775102, 21405167), and the State Key Laboratory of Ocean Engineering (Shanghai Jiao Tong University), China (No. 1609).

Metallothionein (MT) is a low-molecular-weight protein with high inducibility and binding ability with metal ions. Therefore, MT is often regarded as an important biomarker for assessment of heavy metal pollution in water environment. But the traditional process of its enrichment and identification is time-consuming and complicated. Herein, we prepared a core-shell nanoparticle, gold-coated iron oxide nanoparticles (Fe₃O₄@Au NPs). The nanoparticle possessed the advantages such as fast response to magnetic fields and optical properties attributing to Fe₃O₄ and Au nanoparticles separately. Fe₃O₄@Au nanoparticles were used to enrich MT simply through AuS interaction, and the purified proteins were determined by matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF/MS). The results in this work showed that the Fe₃O₄@Au nanoparticles could directly enrich MT from complex solutions and the detection limit could be down to 10 fg/mL.
- Metallothionein,
- Low-abundant,
- Magnetic nanomaterials,
- Gold nanoparticles,
- Core-shell structure,
- Mass spectrometry
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