Citation: GONG Hang, LIU Bei-Bei, LI Pan, HE Dan, GUO Yi-Rong, WANG Li-Min, HUA Xiu-De, WANG Ming-Hua, LIU Feng-Quan, XU Zheng-Lin, ZHANG Cun-Zheng, WANG Li. Immunochromatographic Assay for Maximal Residual Limit-based Multiple Quantitative Determination of Triazophos in Agro Products[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(6): 938-946. doi: 10.11895/j.issn.0253-3820.171277 shu

Immunochromatographic Assay for Maximal Residual Limit-based Multiple Quantitative Determination of Triazophos in Agro Products

GONG Hang ^1,2, ,
LIU Bei-Bei ² ,
LI Pan ² ,
HE Dan ^1,2 ,
GUO Yi-Rong ³ ,
WANG Li-Min ⁴ ,
HUA Xiu-De ⁴ ,
WANG Ming-Hua ⁴ ,
LIU Feng-Quan ² ,
XU Zheng-Lin ⁵ ,
ZHANG Cun-Zheng ² ,
WANG Li ¹

1.
College of Food and Drug, Anhui Science and Technology University, Fengyang 233100, China;
2.
Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Science, Nanjing 210014, China;
3.
Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou 310058, China;
4.
College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China;
5.
South China Agricultural University, Guangzhou 510642, China

Received Date: 28 September 2017
Revised Date: 24 March 2018

Fund Project: This work was supported by the National Key Research and Development Program of China (No.2016YFD0200803-3), the Key Project of Anhui Provincial Excellent Young Talent Support Program (No. gxyqZD2016219), the Independent Innovation Project of Jiangsu Province (No. CX (17) 1003) and the Key Technology R&D Program of Jiangsu Province (No. BE2014722).

A novel immunochromatographic assay was developed, which could provide visual evidence of triazophos in agro products, and also could directly identify the safety status by setting visual cut-off limit of detection in maximal residual limit (MRL) value. Three test lines (T1, T2, T3) were applied to the nitrocellulose membrane with different concentrations of Triazophos-OVA, and one control line (C) was settled with goat anti mouse IgG antibody. Thereafter, by combining with conjugate pad which immobilized monoclonal antibody labeled with 20 nm Colloidal gold particles, absorbent pad and PVC plate, a chromatographic test strip was assembled. With optimization of sample extraction and solvents selection, the test strips were employed for the determination of triazophos in rice, cabbage and apple. The results revealed that the cut-off limit of detection could reach 0.005, 0.01 and 0.02 μg/mL represented by test line T3, T2 and T1, respectively. After modification, the cut-off limit of detection was resettled to 0.05, 0.1 and 0.2 μg/mL according to the MRL values which enforced by the national standard of GB2763. Using acetonitrile for the sample extraction, the extracts were diluted 10 times or solvent exchanged with equivalent volume by PBS solution, and then tested by strips descripted above mentioned. The two test strips could precisely identified the safety status of agro product with MRL as threshold within 8-12 min. Furthermore, the residues value of triazophos could be quantified by the multiple quantitative test lines. Parallel GC data indicated that the strip had no false negative. This MRL-based multiple quantitative triazophos detection strip would provide a simple, direct, accurate and the most intuitionistic performance for the evaluation of agro product safety.
- Triazophos,
- Immunochromatographic assay,
- Maximal residual limit value,
- Rapid detection
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