Citation: YANG Jie, LI Shuang, WU Xue-Yuan, LONG Yi-Tao. Development of Biological Nanopore Technique in Non-gene Sequencing Application[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(12): 1766-1775. doi: 10.11895/j.issn.0253-3820.171201 shu

Development of Biological Nanopore Technique in Non-gene Sequencing Application

Key Laboratory for Advanced Materials, School of Chemical and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China

Corresponding author: LONG Yi-Tao, ytlong@ecust.edu.cn
Received Date: 27 August 2017
Accepted Date: 10 October 2017

Fund Project: the Fundamental Research Funds for the Central Universities 222201717003the Innovation Program of Shanghai Municipal Education Commission 2017-01-07-00-02-E00023This work was supported by the National Natural Science Foundation of China (Nos. 21421004, 21327807), the Program of Introducing Talents of Discipline to Universities (No. B16017), the Innovation Program of Shanghai Municipal Education Commission (No. 2017-01-07-00-02-E00023) and the Fundamental Research Funds for the Central Universities (Nos. 222201718001, 222201717003)the National Natural Science Foundation of China 21421004the Fundamental Research Funds for the Central Universities 222201718001the National Natural Science Foundation of China 21327807the Program of Introducing Talents of Discipline to Universities B16017

Figures(7)

Nanopore technique is a low-cost, ultrafast method for single-molecule level analysis without labels. Nanopore technique was first proposed more than 20 years ago and exhibited an excellent potential in DNA sequencing. So far, the commercial development of nanopore strand-sequencing as a portable device has been realized. Meanwhile, a remarkable number of studies have demonstrated that nanopore represents versatile single-molecule sensors for a wide range of molecule. Therefore, in this article we mainly review the use of nanopore technique based on the interface interactions between biological pore and the analytes such as protein/peptide to obtain kinetic and thermodynamic information at single-molecule level. And a large number of biological molecules and metal ions are quantitatively detected by nanopore analysis, allowing its development for the future biotechnologies and medicine applications. Besides, electrochemical detection system is crucial to nanopore technique. Therefore, we focus on advancements in relative software and ultralow current instrumentations with high-bandwidth.
- Nanochannel,
- Single-molecule detection,
- Electrochemical analysis,
- Ultralow current amplifier,
- Review
1. [1]
  Kasianowicz J J, Brandin E, Branton D, Deamer D W. Proc. Natl. Acad. Sci. USA, 1996, 93(24):13770-13773 doi: 10.1073/pnas.93.24.13770
2. [2]
  Butler T Z, Pavlenok M, Derrington I M, Niederweis M, Gundlach J H. Proc. Natl. Acad. Sci. USA, 2008, 105(52):20647-20652 doi: 10.1073/pnas.0807514106
3. [3]
  Niederweis M. Mol. Microbiol., 2003, 49(5):1167-1177 doi: 10.1046/j.1365-2958.2003.03662.x
4. [4]
  Wendell D, Jing P, Geng J, Subramaniam V, Lee T J, Montemagno C, Guo P. Nat. Nanotech., 2009, 4(11):765-772 doi: 10.1038/nnano.2009.259
5. [5]
  Soskine M, Biesemans A, De Maeyer M, Maglia G. J.Am.Chem.Soc., 2013, 135(36):13456-13463 doi: 10.1021/ja4053398
6. [6]
  Mohammad M M, Iyer R, Howard K R, Mcpike M P, Borer P N, Movileanu L. J. Am.Chem.Soc., 2012, 134(22):9521-9531 doi: 10.1021/ja3043646
7. [7]
  Aoki T, Hirano M, Takeuchi Y, Kobayashi T, Yanagida T, Ide T. P. Jpn. Acad. B, 2010, 86(9):920-925 doi: 10.2183/pjab.86.920
8. [8]
  Goyal P, Krasteva P V, Van Gerven N, Gubellini F, Van Den Broeck I, Troupiotis-Tsailaki A, Jonckheere W, Pehau-Arnaudet G, Pinkner J S, Chapman M R, Hultgren S J, Howorka S, Fronzes R, Remaut H. Nature, 2014, 516(7530):250-253 doi: 10.1038/nature13768
9. [9]
  Wang H Y, Li Y, Qin L X, Heyman A, Shoseyov O, Willner I, Long Y T, Tian H. Chem. Commun., 2013, 49(17):1741-1743 doi: 10.1039/c3cc38939a
10. [10]
  Wang Y, Montana V, Grubišić V, Stout R F, Parpura V, Gu L Q. ACS Appl. Mater. Interfaces, 2015, 7(1):184-192 doi: 10.1021/am5056596
11. [11]
  Cao C, Ying Y L, Hu Z L, Liao D F, Tian H, Long Y T. Nat. Nanotech., 2016, 11(8):713-718 doi: 10.1038/nnano.2016.66
12. [12]
  Cao C, Yu J, Wang Y Q, Ying Y L, Long Y T. Anal. Chem., 2016, 88(10):5046-5049 doi: 10.1021/acs.analchem.6b01514
13. [13]
  CAO Chan, LIAO Dong-Fang, YING Yi-Lun, LONG Yi-Tao. Acta Chimica Sinica, 2016, 74(9):734-737
14. [14]
  Cao C, Liao D F, Yu J, Long Y T. Nat. Protoc., 2017, 12(9):1901 doi: 10.1038/nprot.2017.077
15. [15]
  Cao C, Yu J, Li M Y, Wang Y Q, Tian H, Long Y T. Small, 2017, DOI:10.1002/smll.201702011 doi: 10.1002/smll.201702011
16. [16]
  Storm A J, Chen J H, Ling X S, Zandbergen H W, Dekker C. J. Appl. Phys., 2005, 98(1):014307 doi: 10.1063/1.1947391
17. [17]
  Lin Y, Shi X, Liu S C, Ying Y L, Li Q, Gao R, Fathi F, Long Y T, Tian H. Chem. Commun., 2017, 53(25):3539-3542 doi: 10.1039/C7CC00060J
18. [18]
  Shi X, Gao R, Ying Y L, Si W, Chen Y F, Long Y T. ACS Sensors, 2016, 1(9):1086-1090 doi: 10.1021/acssensors.6b00408
19. [19]
  Shi X, Gao R, Ying Y L, Si W, Chen Y, Long Y T. Faraday Discuss., 2015, 184:85-99 doi: 10.1039/C5FD00060B
20. [20]
  Ruby Dela T, Joseph L, Alon S, Amit M. Nanotechnology, 2012, 23(38):385308 doi: 10.1088/0957-4484/23/38/385308
21. [21]
  Venkatesan B M, Dorvel B, Yemenicioglu S, Watkins N, Petrov I, Bashir R. Adv. Mater., 2009, 21(27):2771-2776 doi: 10.1002/adma.200803786
22. [22]
  Garaj S, Hubbard W, Reina A, Kong J, Branton D, Golovchenko J A. Nature, 2010, 467(7312):190-193 doi: 10.1038/nature09379
23. [23]
  Traversif, Raillonc, Benameur S M, Liuk, Khlybovs, Tosunm, Krasnozhond, Kisa, Radenovica. Nat. Nanotech., 2013, 8(12):939-945 doi: 10.1038/nnano.2013.240
24. [24]
  Li J, Stein D, Mcmullan C, Branton D, Aziz M J, Golovchenko J A. Nature, 2001, 412(6843):166-169 doi: 10.1038/35084037
25. [25]
  Wanunu M, Dadosh T, Ray V, Jin J, McReynolds L, Drndić M. Nat. Nanotech., 2010, 5(11):807-814 doi: 10.1038/nnano.2010.202
26. [26]
  Chen P, Mitsui T, Farmer D B, Golovchenko J, Gordon R G, Branton D. Nano Lett., 2004, 4(7):1333-1337 doi: 10.1021/nl0494001
27. [27]
  Zhang S, Sun T, Wang E, Wang J. Chin. Sci. Bull., 2014, 59(35):4946-4952 doi: 10.1007/s11434-014-0626-6
28. [28]
  Jiang Y, Liu N, Guo W, Xia F, Jiang L. J. Am. Chem. Soc., 2012, 134(37):15395-15401 doi: 10.1021/ja3053333
29. [29]
  Li P, Xie G, Kong X Y, Zhang Z, Xiao K, Wen L, Jiang L. Angew. Chem. Int. Ed., 2016, 55(50):15637-15641 doi: 10.1002/anie.201609161
30. [30]
  Venkatesan B M, Bashir R. Nat. Nanotech., 2011, 6(10):615-624 doi: 10.1038/nnano.2011.129
31. [31]
  Ying Y L, Zhang J, Gao R, Long Y T. Angew. Chem. Int. Ed., 2013, 52(50):13154-13161 doi: 10.1002/anie.201303529
32. [32]
  Gao R, Ying Y L, Yan B Y, Long Y T. Chin. Sci. Bull., 2014, 59(35):4968-4973 doi: 10.1007/s11434-014-0656-0
33. [33]
  Guo Y, Jian F, Kang X. RSC Adv., 2017, 7(25):15315-15320 doi: 10.1039/C7RA00454K
34. [34]
  Yao F, Zhang Y, Wei Y, Kang X F. Chem. Commun., 2014, 50(89):13853-13856 doi: 10.1039/C4CC06135G
35. [35]
  Xi D, Shang J, Fan E, You J, Zhang S, Wang H. Anal. Chem., 2016, 88(21):10540-10546 doi: 10.1021/acs.analchem.6b02620
36. [36]
  Zhao Q, De Zoysa R S S, Wang D, Jayawardhana D A, Guan X. J. Am. Chem. Soc., 2009, 131(18):6324-6325 doi: 10.1021/ja9004893
37. [37]
  Sutherland T C, Long Y T, Stefureac R I, Bediako Amoa I, Kraatz H B, Lee J S. Nano Lett., 2004, 4(7):1273-1277 doi: 10.1021/nl049413e
38. [38]
  Long Y, Zhang M. Sci. China, Ser. B:Chem., 2009, 52(6):731-733 doi: 10.1007/s11426-009-0078-z
39. [39]
  Movileanu L, Schmittschmitt J P, Martin Scholtz J, Bayley H. Biophys. J., 2005, 89(2):1030-1045 doi: 10.1529/biophysj.104.057406
40. [40]
  Mohammad M M, Prakash S, Matouschek A, Movileanu L. J. Am. Chem. Soc., 2008, 130(12):4081-4088 doi: 10.1021/ja710787a
41. [41]
  Merstorf C, Cressiot B, Pastoriza Gallego M, Oukhaled A, Betton J M, Auvray L, Pelta J. ACS Chem. Biol., 2012, 7(4):652-658 doi: 10.1021/cb2004737
42. [42]
  Mereuta L, Asandei A, Seo C H, Park Y, Luchian T. ACS Appl. Mater. Interfaces, 2014, 6(15):13242-13256 doi: 10.1021/am5031177
43. [43]
  Mereuta L, Roy M, Asandei A, Lee J, Park Y, Andricioaei I, Luchian T. Sci. Rep., 2014, 4:3885
44. [44]
  Asandei A, Chinappi M, Kang H K, Seo C H, Mereuta L, Park Y, Luchian T. ACS Appl. Mater. Interfaces, 2015, 7(30):16706-16714 doi: 10.1021/acsami.5b04406
45. [45]
  Rodriguez-Larrea D, Bayley H. Nat. Nanotech., 2013, 8(4):288-295 doi: 10.1038/nnano.2013.22
46. [46]
  Wang H Y, Gu Z, Cao C, Wang J, Long Y T. Anal. Chem., 2013, 85(17):8254-8261 doi: 10.1021/ac401496x
47. [47]
  Wang H Y, Ying Y L, Li Y, Kraatz H B, Long Y T. Anal. Chem., 2011, 83(5):1746-1752 doi: 10.1021/ac1029874
48. [48]
  Hu Y X, Ying Y L, Gu Z, Cao C, Yan B Y, Wang H F, Long Y T. Chem. Commun., 2016, 52(32):5542-5545 doi: 10.1039/C6CC01292B
49. [49]
  Wang Y, Montana V, Grubisic V, Stout R F, Jr., Parpura V, Gu L Q. ACS Appl. Mater. Interfaces, 2015, 7(1):184-192 doi: 10.1021/am5056596
50. [50]
  Movileanu L, Howorka S, Braha O, Bayley H. Nat. Biotech., 2000, 18(10):1091-1095 doi: 10.1038/80295
51. [51]
  Wei R, Gatterdam V, Wieneke R, Tampe R, Rant U. Nat. Nanotech., 2012, 7(4):257-263 doi: 10.1038/nnano.2012.24
52. [52]
  Uram J D, Ke K, Hunt A J, Mayer M. Small, 2006, 2(8-9):967-972 doi: 10.1002/(ISSN)1613-6829
53. [53]
  Takakura T, Yanagi I, Goto Y, Ishige Y, Kohara Y. Appl. Phys. Lett., 2016, 108(12):123701 doi: 10.1063/1.4944641
54. [54]
  Jung J Y, Thornton T J, Chiari M, Kim T H. Jpn. J. Appl. Phys, 2011, 50:127002 doi: 10.7567/JJAP.50.127002
55. [55]
  Schibel A E, Ervin E N. Langmuir, 2014, 30(37):11248-11256 doi: 10.1021/la502714b
56. [56]
  Wang H Y, Song Z Y, Zhang H S, Chen S P. Microchim. Acta, 2016, 183(3):1003-1010 doi: 10.1007/s00604-015-1699-x
57. [57]
  Shim J W, Tan Q, Gu L Q. Nucleic Acids Res., 2009, 37(3):972-982 doi: 10.1093/nar/gkn968
58. [58]
  Ying Y L, Wang H Y, Sutherland T C, Long Y T. Small, 2011, 7(1):87-94 doi: 10.1002/smll.v7.1
59. [59]
  Zhang X, Zhang J, Ying Y L, Tian H, Long Y T. Chem. Sci., 2014, 5(7):2642-2646 doi: 10.1039/c4sc00134f
60. [60]
  Rotem D, Jayasinghe L, Salichou M, Bayley H. J. Am. Chem. Soc., 2012, 134(5):2781-2787 doi: 10.1021/ja2105653
61. [61]
  Zhang L, Zhang K, Liu G, Liu M, Liu Y, Li J. Anal. Chem., 2015, 87(11):5677-5682 doi: 10.1021/acs.analchem.5b00791
62. [62]
  Kawano R, Osaki T, Sasaki H, Takinoue M, Yoshizawa S, Takeuchi S. J. Am. Chem. Soc., 2011, 133(22):8474-8477 doi: 10.1021/ja2026085
63. [63]
  Wen S, Zeng T, Liu L, Zhao K, Zhao Y, Liu X, Wu H C. J. Am. Chem. Soc., 2011, 133(45):18312-18317 doi: 10.1021/ja206983z
64. [64]
  Yang C, Liu L, Zeng T, Yang D, Yao Z, Zhao Y, Wu H C. Anal. Chem., 2013, 85(15):7302-7307 doi: 10.1021/ac401198d
65. [65]
  Ying Y L, Zhang J, Meng F N, Cao C, Yao X, Willner I, Tian H, Long Y T. Sci. Rep., 2013, 3:1662 doi: 10.1038/srep01662
66. [66]
  Meng F N, Yao X, Ying Y L, Zhang J, Tian H, Long Y T. Chem. Commun., 2015, 51(7):1202-1205 doi: 10.1039/C4CC07919A
67. [67]
  Li T, Liu L, Li Y, Xie J, Wu H C. Angew. Chem. Int. Ed., 2015, 54(26):7568-7571 doi: 10.1002/anie.201502047
68. [68]
  DUAN Jing, ZHUO Sha, YAO Fu-Jun, ZHANG Ya-Ni, KANG Xiao-Feng. Chinese J. Anal. Chem., 2016, 44(12):1801-1807
69. [69]
  Shin S H, Bayley H. J. Am. Chem. Soc., 2005, 127(30):10462-10463 doi: 10.1021/ja052194u
70. [70]
  Lee J, Bayley H. Proc. Natl. Acad. Sci. USA, 2015, 112(45):13768-13773 doi: 10.1073/pnas.1510565112
71. [71]
  Rosenstein J K, Wanunu M, Merchant C A, Drndic M, Shepard K L. Nat. Methods, 2012, 9(5):487-492 doi: 10.1038/nmeth.1932
72. [72]
  YAN Bing-Yong, GU Zhen, GAO Rui, CAO Chan, YING Yi-Lun, MA Wei, LONG Yi-Tao. Chinese J. Anal. Chem., 2015, 43(7):971-976 doi: 10.11895/j.issn.0253-3820.150159
73. [73]
  Zhang J, Liu X, Ying Y L, Gu Z, Meng F N, Long Y T. Nanoscale, 2017, 9(10):3458-3465 doi: 10.1039/C6NR09135K
74. [74]
  Gu Z, Ying Y L, Cao C, He P, Long Y T. Anal. Chem., 2015, 87(2):907-913 doi: 10.1021/ac5028758
75. [75]
  Dunbar W B. Anal. Chem., 2015, 87(20):10650-10652 doi: 10.1021/acs.analchem.5b02281
76. [76]
  Gu Z, Ying Y L, Yan B Y, Wang H F, He P G, Long Y T. Chin. Chem. Lett., 2014, 25(7):1029-1032 doi: 10.1016/j.cclet.2014.05.009
1. [1]
  Chenghe Yang , Yi Lü , Rui Liu . The Rise to Fame of Digital PCR. University Chemistry, 2025, 40(4): 340-345. doi: 10.12461/PKU.DXHX202406111
2. [2]
  Zhengli Hu , Jia Wang , Yi-Lun Ying , Shaochuang Liu , Hui Ma , Wenwei Zhang , Jianrong Zhang , Yi-Tao Long . Exploration of Ideological and Political Elements in the Development History of Nanopore Electrochemistry. University Chemistry, 2024, 39(8): 344-350. doi: 10.3866/PKU.DXHX202401072
3. [3]
  Tiejin Chen , Xiaokuang Xue , Jian Li , Minhui Cui , Yongliang Hao , Mianqi Xue , Haihua Xiao , Jiechao Ge , Pengfei Wang . Membrane-anchoring nanoengineered carbon dots as a pyroptosis amplifier for robust tumor photodynamic-immunotherapy. Acta Physico-Chimica Sinica, 2025, 41(10): 100113-0. doi: 10.1016/j.actphy.2025.100113
4. [4]
  Jingwen Wang , Minghao Wu , Xing Zuo , Yaofeng Yuan , Yahao Wang , Xiaoshun Zhou , Jianfeng Yan . Advances in the Application of Electrochemical Regulation in Investigating the Electron Transport Properties of Single-Molecule Junctions. University Chemistry, 2025, 40(3): 291-301. doi: 10.12461/PKU.DXHX202406023
5. [5]
  Zhuomin Zhang , Lanrui Yang , Baorong Zhang , Gongke Li . 化学分析全英课程思政建设初探. University Chemistry, 2025, 40(8): 58-65. doi: 10.12461/PKU.DXHX202410010
6. [6]
  Jiarong Feng , Yejie Duan , Chu Chu , Dezhen Xie , Qiu'e Cao , Peng Liu . Preparation and Application of a Streptomycin Molecularly Imprinted Electrochemical Sensor: A Suggested Comprehensive Analytical Chemical Experiment. University Chemistry, 2024, 39(8): 295-305. doi: 10.3866/PKU.DXHX202401016
7. [7]
  Fangqi Yang . Teaching Practice and Reflection on Contact Angle Measurement Instrument in Material Chemistry Analysis. University Chemistry, 2025, 40(11): 397-401. doi: 10.12461/PKU.DXHX202412008
8. [8]
  Cen Zhou , Biqiong Hong , Yiting Chen . Application of Electrochemical Techniques in Supramolecular Chemistry. University Chemistry, 2025, 40(3): 308-317. doi: 10.12461/PKU.DXHX202406086
9. [9]
  Weigang Zhu , Xiaofei Ma , Yun Tian , Huaji Liu , Fanli Lu , Yalu Ma . 基于知识图谱的“无机化学与化学分析”课程信息化教学资源构建与应用研究. University Chemistry, 2025, 40(6): 9-15. doi: 10.12461/PKU.DXHX202408113
10. [10]
  Tingting Jiang , Jing Chang . Application of Ideological and Political Education in Chemical Analysis Experiment under the Background of Emerging Engineering Education: Taking the Redox Titration Experiment as an Example. University Chemistry, 2024, 39(2): 168-174. doi: 10.3866/PKU.DXHX202308007
11. [11]
  Liwei Wang , Guangran Ma , Li Wang , Fugang Xu . A Comprehensive Analytical Chemistry Experiment: Colorimetric Detection of Vitamin C Using Nanozyme and Smartphone. University Chemistry, 2024, 39(8): 255-262. doi: 10.3866/PKU.DXHX202312094
12. [12]
  Qingtang ZHANG , Xiaoyu WU , Zheng WANG , Xiaomei WANG . Performance of nano Li₂FeSiO₄/C cathode material co-doped by potassium and chlorine ions. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1689-1696. doi: 10.11862/CJIC.20240115
13. [13]
  Lutian Zhao , Yangge Guo , Liuxuan Luo , Xiaohui Yan , Shuiyun Shen , Junliang Zhang . Electrochemical Synthesis for Metallic Nanocrystal Electrocatalysts: Principle, Application and Challenge. Acta Physico-Chimica Sinica, 2024, 40(7): 2306029-0. doi: 10.3866/PKU.WHXB202306029
14. [14]
  Jiahong ZHENG , Jingyun YANG . Preparation and electrochemical properties of hollow dodecahedral CoNi₂S₄ supported by MnO₂ nanowires. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1881-1891. doi: 10.11862/CJIC.20240170
15. [15]
  Chaolin Mi , Yuying Qin , Xinli Huang , Yijie Luo , Zhiwei Zhang , Chengxiang Wang , Yuanchang Shi , Longwei Yin , Rutao Wang . Galvanic Replacement Synthesis of Graphene Coupled Amorphous Antimony Nanoparticles for High-Performance Sodium-Ion Capacitor. Acta Physico-Chimica Sinica, 2024, 40(5): 2306011-0. doi: 10.3866/PKU.WHXB202306011
16. [16]
  Yang Meiqing , Lu Wang , Haozi Lu , Yaocheng Yang , Song Liu . Recent Advances of Functional Nanomaterials for Screen-Printed Photoelectrochemical Biosensors. Acta Physico-Chimica Sinica, 2025, 41(2): 100018-0. doi: 10.3866/PKU.WHXB202310046
17. [17]
  Xue Dong , Xiaofu Sun , Shuaiqiang Jia , Shitao Han , Dawei Zhou , Ting Yao , Min Wang , Minghui Fang , Haihong Wu , Buxing Han . Electrochemical CO₂ Reduction to C₂₊ Products with Ampere-Level Current on Carbon-Modified Copper Catalysts. Acta Physico-Chimica Sinica, 2025, 41(3): 100024-0. doi: 10.3866/PKU.WHXB202404012
18. [18]
  Shengbiao Zheng , Liang Li , Nini Zhang , Ruimin Bao , Ruizhang Hu , Jing Tang . Metal-Organic Framework-Derived Materials Modified Electrode for Electrochemical Sensing of Tert-Butylhydroquinone: A Recommended Comprehensive Chemistry Experiment for Translating Research Results. University Chemistry, 2024, 39(7): 345-353. doi: 10.3866/PKU.DXHX202310096
19. [19]
  Hong LI , Xiaoying DING , Cihang LIU , Jinghan ZHANG , Yanying RAO . Detection of iron and copper ions based on gold nanorod etching colorimetry. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 953-962. doi: 10.11862/CJIC.20230370
20. [20]
  Jinghan ZHANG , Guanying CHEN . Progress in the application of rare-earth-doped upconversion nanoprobes in biological detection. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2335-2355. doi: 10.11862/CJIC.20240249

Get Citation

PDF

XML

Metrics

PDF Downloads(19)
Abstract views(1911)
HTML views(303)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142