Citation: SHEN Hui-Hui, CHEN Jun-Hui, XU Xiu-Li, PAN Lei, HE Xiu-Ping, WANG Xiao-Ru. Development of A High Performance Liquid Chromatography-Tandem Mass Spectrometry Method for Determination of Lipophilic Toxins in Marine Shellfishes and Edible Safety Evaluation[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(6): 985-992. doi: 10.11895/j.issn.0253-3820.171162 shu

Development of A High Performance Liquid Chromatography-Tandem Mass Spectrometry Method for Determination of Lipophilic Toxins in Marine Shellfishes and Edible Safety Evaluation

SHEN Hui-Hui ^1,2, ,
CHEN Jun-Hui ^2,3 ,
XU Xiu-Li ¹ ,
PAN Lei ² ,
HE Xiu-Ping ² ,
WANG Xiao-Ru ^2,3

1.
School of Ocean Sciences, China University of Geosciences, Beijing 100083, China;
2.
Research Center for Marine Ecology, the First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China;
3.
Xiamen Huaxia University, Xiamen 361024, China

Received Date: 10 August 2017
Revised Date: 28 March 2018

Fund Project: This work was supported by the Basic Scientific Fund for National Public Research Institutes of China (Nos. 2018Q08, 2016S02) and the NSFC-Shandong Joint Fund for Marine Ecology and Environmental Sciences (Nos. U1706217, U1606404).

In view of the present situation that edible marine shellfishes are combinedly contaminated by different kinds of lipophilic toxins, common lipophilic shellfish toxins in marine shellfishes were simultaneously detected by liquid chromatography-tandem mass spectrometry, and the safety risk of commercial marine shellfish was evaluated using the risk assessment method based on combined contamination of various toxins. Under the optimum conditions, satisfactory recoveries (63.3%-88.8%), precision (relative standard deviations RSD ≤ 14.5%) and sensitivity (limit of detection in the range of 0.5-2.7 ng/g) of the method were achieved for all the analytes. Among the 105 commercially available shellfish samples, 42.86% of the samples had at least a kind of toxin. The highest average content was 47.6 μg/kg of DTX1, which was the most serious contaminant for marine shellfishes. The total Expose Risk Index (ΣERI) was calculated based on Tolerable Daily Intake (TDI) and Acute Reference Dose (ARfD) of each toxin to evaluate the safety risk of commercial marine shellfish. The results showed that the ratio of commercially available marine shellfish with safety risk was 19.05% and the food safety risk of scallop was the highest. In summary, a new method based on the combined contamination of lipophilic shellfish toxins was successfully developed for risk assessment of the commercial marine shellfish. The proposed method is more harsh compared with the European Food Safety Authority (EFSA) regulation and can make shellfish consumers better to avoid the risk of poisoning.
- Liquid chromatography-tandem mass spectrometry,
- Marine shellfishes,
- Lipophilic shellfish toxins,
- Combined contamination,
- Food safety
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