Citation: DING Bo, WANG Zhi-Yuan, CHEN Wen-Rui, XIE Jian-Jun, SHE Zhi-Gang. Identification of Natural Caffeine Source in Coffee Drinks by Liquid Chromatography Coupled with Isotope Ratio Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(11): 1802-1807. doi: 10.11895/j.issn.0253-3820.171101 shu

Identification of Natural Caffeine Source in Coffee Drinks by Liquid Chromatography Coupled with Isotope Ratio Mass Spectrometry

DING Bo ^1,2,3, ,
WANG Zhi-Yuan ^1,2 ,
CHEN Wen-Rui ^1,2 ,
XIE Jian-Jun ^1,2 ,
SHE Zhi-Gang ³

¹ Guangdong Inspection and Quarantine Technology Center, Guangzhou 510623, China;
² Guangdong Key Laboratory of Import and Export technical Measures of Animal, Plant and Food, Guangzhou 510623, China;
³ School of Chemistry, Sun Yat-sen University, Guangzhou 510275, China

Received Date: 17 July 2017
Revised Date: 22 September 2018

Fund Project: This work was supported by the Science & Technology Plan Project of Guangdong Province of China (No. 2016A040403091) and the Guangdong Key Laboratory of Import and Export Technical Measures of Animal, Plant and Food Project of Science & Technology Planning (No. 2017A09).

Coffee and coffee drinks are products preferred by a number of consumers in their daily life. However, it is lack of viable methods for identification of natural coffee source. A new method of liquid chromatography coupled to isotope ratio mass spectrometry (LC-IRMS) was developed for determination of caffeine δ¹³C value for discrimination of natural and synthetic caffeine source in the coffee drinks. Caffeine in samples was separated by reversed-phase liquid chromatography with An XBridge Shild PR C₁₈ (2.1 mm×50 mm, 3.5 μm). The column temperature was 50℃ and the water flow rate was 400 μL/min. Target analytes were converted to CO₂ in the oxidation reactor of the LC-IsoLink interface. Finally the caffeine δ¹³C value was directly detected with a Delta V Advantage isotope ratio mass spectrometer. On the basis of the carbon isotope analysis of 44 coffee soybean samples by LC-IRMS, it was concluded that there was one distinguishable group of δ¹³C-values:-25.3‰ to -30.9‰ in natural coffee soybean. The average of δ¹³C-values (δ_average) was -27.9 ‰, and the standard deviation (s) was 1.3‰. The samples were discriminated for unnatural coffee soybean source, when the caffeine δ¹³C-value was less than or approached δ_average-3s (-31.7‰). This method was applied to identify the caffeine source in 16 kinds of coffee drinks, in which one sample was discriminated to have unnatural coffee soybean source.
- Liquid chromatography coupled to isotope ratio mass spectrometry,
- Coffee drinks,
- Caffeine
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
15. [15]
16. [16]
17. [17]
18. [18]
19. [19]
20. [20]
21. [21]
22. [22]
23. [23]
24. [24]
25. [25]
26. [26]
27. [27]
1. [1]
  Longping Li , Jiali Li , Tiange Qu , Jiaqing Cai , Chuyu Zhang , Wenji Guo , Qiulian Li , Fan Luo . “可视化”助力从茶叶中提取咖啡因实验的关键步——升华. University Chemistry, 2025, 40(8): 272-276. doi: 10.12461/PKU.DXHX202409137
2. [2]
  Zhanming Zhang , Can Zhu , Juan Wang , Yanghui Lin , Mo Sun . Ideological and Political Cases in the Course of Organic Chemistry Experiment: Taking Caffeine Extraction from Tea Leaves Experiment as an Example. University Chemistry, 2025, 40(7): 34-41. doi: 10.12461/PKU.DXHX202409030
3. [3]
  Yifan Xie , Liyun Yao , Ruolin Yang , Yuxing Cai , Yujie Jin , Ning Li . Application of Comparative Pedagogy in Instrumental Analysis Experiment Teaching. University Chemistry, 2024, 39(3): 266-273. doi: 10.3866/PKU.DXHX202309068
4. [4]
  Tong Zhou , Liyi Xie , Chuyu Liu , Xiyan Zheng , Bao Li . Between Sobriety and Intoxication: The Fascinating Journey of Sauce-Flavored Latte. University Chemistry, 2024, 39(9): 55-58. doi: 10.12461/PKU.DXHX202312048
5. [5]
  Zongyuan Chen , ChunSheng Shi , Yiwen Li , Ganlin Zu , Qiang Jin , Haishan Wang , Fujun Wang , Dekun Yan , Zhijun Guo , Wangsuo Wu . Measurement of Uranium Isotopes in Environmental Water Samples by Alpha-Spectroscopy: Design of an Undergraduate Radiochemistry Experiment. University Chemistry, 2025, 40(4): 353-358. doi: 10.12461/PKU.DXHX202406103
6. [6]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
7. [7]
  Yifan Xie , Liyun Yao , Ruolin Yang , Yuxing Cai , Yujie Jin , Ning Li . Exploration and Practice of Online and Offline Hybrid Teaching Mode in High-Performance Liquid Chromatography Experiment. University Chemistry, 2025, 40(11): 100-107. doi: 10.12461/PKU.DXHX202412133
8. [8]
  Yaling Chen . Basic Theory and Competitive Exam Analysis of Dynamic Isotope Effect. University Chemistry, 2024, 39(8): 403-410. doi: 10.3866/PKU.DXHX202311093
9. [9]
  Wenwen Zhang , Peichao Zhang , Conghao Gai , Xiaoyun Chai , Yan Zou , Qingjie Zhao . Unveiling Kinetics at Natural Abundance: ¹³C NMR Isotope Effect Experiments. University Chemistry, 2025, 40(10): 203-207. doi: 10.12461/PKU.DXHX202411076
10. [10]
  Runjie Li , Hang Liu , Xisheng Wang , Wanqun Zhang , Wanqun Hu , Kaiping Yang , Qiang Zhou , Si Liu , Pingping Zhu , Wei Shao . 氨基酸的衍生及手性气相色谱分离创新实验. University Chemistry, 2025, 40(6): 286-295. doi: 10.12461/PKU.DXHX202407059
11. [11]
  Feng Sha , Xinyan Wu , Ping Hu , Wenqing Zhang , Xiaoyang Luan , Yunfei Ma . Design of Course Ideology and Politics for the Comprehensive Organic Synthesis Experiment of Benzocaine. University Chemistry, 2024, 39(2): 110-115. doi: 10.3866/PKU.DXHX202307082
12. [12]
  Chongjing Liu , Yujian Xia , Pengjun Zhang , Shiqiang Wei , Dengfeng Cao , Beibei Sheng , Yongheng Chu , Shuangming Chen , Li Song , Xiaosong Liu . Understanding Solid-Gas and Solid-Liquid Interfaces through Near Ambient Pressure X-Ray Photoelectron Spectroscopy. Acta Physico-Chimica Sinica, 2025, 41(2): 100013-0. doi: 10.3866/PKU.WHXB202309036
13. [13]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
14. [14]
  Shunü Peng , Huamin Li , Zhaobin Chen , Yiru Wang . Simultaneous Application of Multiple Quantitative Analysis Methods in Gas Chromatography for the Determination of Active Ingredients in Traditional Chinese Medicine Preparations. University Chemistry, 2025, 40(10): 243-249. doi: 10.12461/PKU.DXHX202412043
15. [15]
  Guodong Xu , Chengcai Sheng , Xiaomeng Zhao , Tuojiang Zhang , Zongtang Liu , Jun Dong . Reform of Comprehensive Organic Chemistry Experiments in the Context of Emerging Engineering Education: A Case Study on the Improved Preparation of Benzocaine. University Chemistry, 2024, 39(11): 286-295. doi: 10.12461/PKU.DXHX202403094
16. [16]
  Yongzhi LI , Han ZHANG , Gangding WANG , Yanwei SUI , Lei HOU , Yaoyu WANG . A two-dimensional metal-organic framework for the determination of nitrofurantoin and nitrofurazone in aqueous solution. Chinese Journal of Inorganic Chemistry, 2025, 41(2): 245-253. doi: 10.11862/CJIC.20240307
17. [17]
  Mingyang Men , Jinghua Wu , Gaozhan Liu , Jing Zhang , Nini Zhang , Xiayin Yao . Sulfide Solid Electrolyte Synthesized by Liquid Phase Approach and Application in All-Solid-State Lithium Batteries. Acta Physico-Chimica Sinica, 2025, 41(1): 100004-0. doi: 10.3866/PKU.WHXB202309019
18. [18]
  Yajie Li , Bin Chen , Yiping Wang , Hui Xing , Wei Zhao , Geng Zhang , Siqi Shi . Inhibiting Dendrite Growth by Customizing Electrolyte or Separator to Achieve Anisotropic Lithium-Ion Transport: A Phase-Field Study. Acta Physico-Chimica Sinica, 2024, 40(3): 2305053-0. doi: 10.3866/PKU.WHXB202305053
19. [19]
  Tong WANG , Xuefang ZHU , Qi GAO , Hongbo ZHANG , Chao REN , Lixia GE . Luminescence and thermal stability of Tb³⁺-Eu³⁺ doped glass-ceramics containing Na_8.12Y_1.293Si₆O₁₈ crystal phase. Chinese Journal of Inorganic Chemistry, 2025, 41(11): 2237-2250. doi: 10.11862/CJIC.20250137
20. [20]
  Yu Peng , Jiawei Chen , Yue Yin , Yongjie Cao , Mochou Liao , Congxiao Wang , Xiaoli Dong , Yongyao Xia . Tailored cathode electrolyte interphase via ethylene carbonate-free electrolytes enabling stable and wide-temperature operation of high-voltage LiCoO₂. Acta Physico-Chimica Sinica, 2025, 41(8): 100087-0. doi: 10.1016/j.actphy.2025.100087

Get Citation

PDF

XML

Metrics

PDF Downloads(23)
Abstract views(1118)
HTML views(165)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142