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Citation: DENG Wen-Chan, HAN Guo-Bin, LI Yuan-Fang, HUANG Cheng-Zhi. Distinction of Cells Infected with Respiratory Syncytial Virus by Matrix Assisted Laser Desorption/Ionization Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(2): 165-169. doi: 10.11895/j.issn.0253-3820.171011 shu

Distinction of Cells Infected with Respiratory Syncytial Virus by Matrix Assisted Laser Desorption/Ionization Mass Spectrometry

1.
Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China;
2.
College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China

Received Date: 16 June 2017
Revised Date: 12 December 2017

Fund Project: This work was supported by the National Natural Science Foundation of China (No.21535006).

Respiratory syncytial virus (RSV), which is a major cause of lower respiratory tract infections during infancy and childhood, is also an important pathogen for immunosuppression in elder and diseased areas. In order to research the pathogenesis of RSV, the matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS)was used to explore the difference between uninfected cells and virus-infected cells. The result showed that, in the molecular weight range of 5000~10000 Da, there are three component peaks expressed with significant difference in both normal group and infection group. One of the three components was up-regulated (m/z 6154.25) and the other two were down-regulated (m/z 7658.47 and 9259.82) after RSV infection. This result proved the obvious differences between the infected cells and untreated cells.The differential expression may provide a feasible method for RSV disease diagnosis and pathology research, and also provide scientific evidences for research on development of RSV therapeutic drugs.
- Matrix-assisted laser desorption/ionization mass spectrometry,
- Respiratory syncytial virus,
- Biomarker
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