Citation: JIANG Xiao-Xiao, WANG Jiang, GUAN Qi-Yuan, HU Jun, XU Jing-Juan, CHEN Hong-Yuan. Identification of C＝C Location of Unsaturated Phosphatidylcholines in Cell by Photochemical Reaction-Tandem Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(12): 1988-1995. doi: 10.11895/j.issn.0253-3820.170350 shu

Identification of C＝C Location of Unsaturated Phosphatidylcholines in Cell by Photochemical Reaction-Tandem Mass Spectrometry

State Key Laboratory of Analytical Chemistry for Life Science in Nanjing University, Nanjing 210023, China

Corresponding author: XU Jing-Juan, xujj@nju.edu.cn
Received Date: 3 June 2017
Accepted Date: 28 September 2017

Fund Project: the National Natural Science Foundation of China 21327902This work was supported by the National Natural Science Foundation of China (No. 21327902)

Figures(4)

A method of identification of C＝C location and relative quantitation of unsaturated phosphatidylcholine (PC) isomers in breast cells by online photochemical reaction-pulsed directed current electrospray-tandem mass spectrometry (PB-pulsed-dc-ESI-MS/MS) was established with benzophenone (BP) as a photochemical reactant. The three-phase extraction method was used to extract the lipids in the cells, and then the C＝C in the unsaturated PC and the carbonyl in BP were specifically cycled under the irradiation of 254 nm ultraviolet light (Paternò-Büchi, PB reaction). The PB products were ionized and mass-isolated for low-energy collision dissociation through the non-contact pulsed-dc-ESI ionization method. The double bond position and the relative content of the location isomers were obtained from the resulting ions in the MS/MS spectrum. The C＝C location of 8 kinds of unsaturated PCs in MCF-7 and MCF-10A was detected, and the relative contents of 4 kinds of C＝C location isoforms were analyzed. It was found that the relative abundance of Δ9 isomer in PC 16:0_18:1 was not significantly different between the two cells. The relative abundance of Δ9 isomers in PC 18:0_18:1 and PC 18:1_18:1 was slightly different. However, there is a big difference of Δ9 in LPC 18:1 between the cancer cell and normal cell (56.0% ±1.3% vs. 71.7% ±6.8%). The establishment of such a rapid and easy mass spectrometry method can analyze the C＝C location and the relative content of location isomers, and it is expected to be a powerful tool to identify different cell states and different disease states.
- Online photochemical reaction,
- Unsaturated phosphatidylcholines,
- C C location,
- Tandem mass spectrometry
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