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Citation: LIU Lin-Lin, ZHENG Meng-Lin, ZOU Bing-Jie, SONG Qin-Xin, ZHOU Guo-Hua. Highly Sensitive and Specific Detection of SRY Gene for Non-invasive Prenatal Diagnosis[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(10): 1448-1454. doi: 10.11895/j.issn.0253-3820.170332 shu

Highly Sensitive and Specific Detection of SRY Gene for Non-invasive Prenatal Diagnosis

1.
Key Laboratory of Drug Quality Control and Pharmacovigilance of Ministry of Education, China Pharmaceutical University, Nanjing 210009, China;2 Department of pharmacology, Jinling Hospital, Nanjing 210002, China

Received Date: 26 May 2017
Revised Date: 25 July 2017

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 81673390), the Key Research Program of Jiangsu Province of China (Social Development) (No. BE2016745), the Basic Research Program of Jiangsu Province (Natural Science Foundation) (No. BK20151445), the Program of MOE Key Laboratory of Drug Quality Control and Pharmacovigilance of China (No. DQCP2015MS02) and Qing Lan Project of Jiangsu Province of China.

By detecting SRY gene of cell-free fetal DNA (cffDNA) in maternal peripheral blood, the sex of fetuses was determined, the risks of sex-linked genetic disorders were assessed and the birth rate of sick fetuses was decreased. A method of real-time polymerase chain reaction (PCR) coupled with invader assay was established to detect SRY gene. This method possessed the advantages such as high sensitivity, high specificity and non-contaminated with closed tube detection. Under the optimized reaction conditions such as 250 nmol/L detection probes, 7.5 U FEN1 enzyme, 0.5 U Taq polymerase and 67℃ of annealing temperature in pre-amplification, the simulated samples as low as 4‰ (4 copies/μL) were detected and two clinical samples with the gestation age of 9 weeks and 10 weeks were successfully detected. The detection results showed that this method could be used to detect SRY gene of cffDNA in maternal peripheral blood, providing an effective technique for clinical non-invasive prenatal diagnosis based on SRY gene.
- Real-time polymerase chain reaction,
- Invader assay,
- Cell-free fetal DNA,
- SRY gene,
- Non-invasive prenatal diagnosis
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1.
沈阳化工大学材料科学与工程学院沈阳 110142

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