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Citation: YAO Xue-Lian, CHEN Zha-Dan, ZHANG Jing, CUI Han-Feng, LIN Yan, HONG Nian, HE Ling-Ling, KONG De-Rong, FAN Hao, CHENG Lin. Preparation of Thrombin Aptamer Sensor Based on Host Guest Competition Model[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(10): 1421-1426. doi: 10.11895/j.issn.0253-3820.170268 shu

Preparation of Thrombin Aptamer Sensor Based on Host Guest Competition Model

College of Pharmacy, JiangXi University of Traditional Chinese Medicine, Nanchang 330004, China

Received Date: 30 April 2017
Revised Date: 5 August 2017

Fund Project: This work was supported by the National Natural Science Foundation of China (No.81660658)

A switch thrombin aptamer sensor was constructed based on the host-guest competition mode of β-cyclodextrin (β-CD). The aptamer that modified with ferrocene (Fc) at its terminal was fixed on the surface of the gold electrode via the host-guest recognization with β-CD. When thrombin was present, the configuration of aptamer transformed from vertical linear to "G-quadruplex" and far away from the electrode surface, which resulted in a decrease in the redox current of the aptamer probe and produce "Signal-off" effect. On the basis of this, a high sensitive detection of thrombin was made. The detection result indicated that the thrombin concentration had a consideration linear response to the signal of aptasensor between 5.0×10^-13-5.0×10^-9 mol/L, and as low as 2.0×10^-13 mol/L thrombin had been detected. This method for thrombin detection showed a higher specificity compared to other protein molecules. Besides, the sensor was constructed easily and possessed excellent regeneration, which provided a significant platform for highly efficient real-time detection of thrombin in biological serum samples.
- β-Cyclodextrin,
- Host-guest recognition,
- Aptamer,
- Thrombin,
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