Citation: PANG Bo, WANG Bin, SHU Zhen-Bo, YAO Xiao-Xiao, ZHANG Guo-Dong, HU Cong, WU SUI-Sheng. Metabonomics Study of Chronic Heart Failure by Rapid Resolution Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(8): 1165-1171. doi: 10.11895/j.issn.0253-3820.170006 shu

Metabonomics Study of Chronic Heart Failure by Rapid Resolution Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry

PANG Bo ^1,2, ,
WANG Bin ¹ ,
SHU Zhen-Bo ³ ,
YAO Xiao-Xiao ⁴ ,
ZHANG Guo-Dong ¹ ,
HU Cong ¹ ,
WU SUI-Sheng ¹

1.
First Hospital of Jilin University, Changchun 130021, China;2 The 208 th Hospital of PLA, Changchun 130062, China;3 China-Japan Union Hospital, Jilin University, Changchun 130031, China;4 The No.2 Hospital, Jilin University, Changchun 130000, China

Received Date: 4 January 2017
Revised Date: 13 June 2017

In this study, the rapid resolution liquid chromatography-quadrupole time-of-flight mass spectrometry (RRLC-QTOF/MS) was used to profile the metabolites of urine samples from chronic heart failure (CHF) patients and healthy controls to find the differential metabolites which could provide the scientific evidence to explain the pathogenesis of the disease and supply a better therapy plan. Urine samples from 15 CHF patients (age (62.27±3.14) years) and 15 healthy controls (age (65.41±4.63) years) were analyzed by RRLC-QTOF/MS. After processing the data, the multivariate statistical analysis (principal component analysis, PCA) was performed to find the potential biomarkers. Result showed that urine samples of CHF patients were successfully distinguished from those of healthy controls. Two significantly differentially expressed metabolites, uridine and alanyltryptophan, were found and identified as potential biomarkers. The result showed that the LC-MS based metabolomics approach had good performance to identify potential biomarkers, and the disorder of uracil metabolism and Tryptophan metabolism may play an important role in the mechanism of CHFs
- Chronic heart failure,
- Rapid resolution liquid chromatography-quadrupole time-of-flight mass spectrometry,
- Uridine,
- Alanyltryptophan,
- Metabonomics
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