Citation: LIU Chen-Guang, WANG Jiu-Jun, ZHANG Xing-Ping, YANG Hua-Lin. A Fluorescence Biosensor for Detection of Mercury Ion Based on Oligonucleotide[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(2): 163-168. doi: 10.11895/j.issn.0253-3820.160620 shu

A Fluorescence Biosensor for Detection of Mercury Ion Based on Oligonucleotide

College of College of Life Science, Yangtze University, Jingzhou 434025, China

Received Date: 22 August 2016
Revised Date: 17 November 2016

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 31601536)

A simple, fast and highly sensitive fluorescence analysis method for detection of mercury ion was developed based on N-methyl-mesoporphyrin IX(NMM)/G-quadruplex DNA system and specific T-Hg(Ⅱ)-T mismatches. In this strategy, a large number of thymine was introduced into guanine-rich oigonucleotides which could form G-quadruplex. In the presence of Hg²⁺, guanine-rich oigonucleotides and complementary strand could form double-stranded DNA molecule by specific T-Hg(Ⅱ)-T mismatch pair, leading to destruction of G-quadruplex DNA structure. In the absence of Hg²⁺, guanine-rich oigonucleotides spontaneously formed G-quadruplex DNA structure that could bound NMM to generate intense fluorescence. Based on the above facts, a sensitive fluorescence biosensor for determination of Hg²⁺ was fabricated. And the optimal conditions for Hg²⁺ determination were as follows:buffer solution pH of 6.7, 20 mmol/L KCl and 2.5 μmol/L NMM in buffer and incubation for 2 h. Under the optimal conditions, the fluorescence intensity signal change (F₀-F) and the Hg²⁺ concentration exhibited a linear correlation within 50 nmol/L to 1000 nmol/L range with a low detection limit of 22.8 nmol/L (3σ). The biosensor exhibited good selectivity toward common metal ions. The developed method was successfully employed to detect Hg²⁺ in tap water with recovery of 106.1%-107.8%.
- G-Quadruplex deoxyribonucleic acid,
- Thymine-Hg-thymine mismatches,
- Mercury ion,
- Fluorescence Biosensor
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