Citation: REN Ju-Jie, YU Cong-Cong, CUI Min, LI Ya-Qing, WU Cong, JI Xue-Ping. Construction of Hemin Composite Based Electrochemical Sensor for Detection of Peroxynitrite[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(1): 104-110. doi: 10.11895/j.issn.0253-3820.160597 shu

Construction of Hemin Composite Based Electrochemical Sensor for Detection of Peroxynitrite

REN Ju-Jie ^1, ,
YU Cong-Cong ¹ ,
CUI Min ¹ ,
LI Ya-Qing ¹ ,
WU Cong ¹ ,
JI Xue-Ping ²

1.
School of Sciences, Hebei University of Science and Technology, Shijiazhuang, 050018, China;
2.
Department of Medical Chemistry, Hebei University of Science and Technology, Shijiazhuang, 050017, China

Received Date: 11 August 2016
Revised Date: 12 November 2016

A peroxynitrite electrochemical sensor based on hemin/gold nanoparticles/poly(melamine)/multi-walled carbon nanotube composite modifying glassy carbon electrode was successfully constructed for the detection of peroxynitrite. The electrochemical behavior of peroxynitrite on the sensor was investigated by cyclic voltammetry and amperometric i-t curve, and the conditions for preparation of the sensor and detection of peroxynitrite were optimized. The results showed that the amperometric response of the sensor for peroxynitrite reached the maximum when the amount of carbon nanotubes was 5 μL, the deposition time of gold was 20 s, and the working potential was 0.8 V. Under the optimized experimental conditions, the sensor could detect peroxynitrite in a linear range from 1.0×10^-5 mol/L to 3.5×10^-4 mol/L and from 3.5×10^-4 mol/L to 1.1×10^-3 mol/L, with a sensitivity of 0.13 A/(mol/L) and a detection limit of 1.2×10^-7 mol/L (S/N=3).
- Peroxynitrite,
- Hemin,
- Poly(melamine),
- Gold nanoparticles,
- Multi-walled carbon nanotubes,
- Electrochemical sensor
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