Citation: WANG Rui-Guo, SU Xiao-Ou, WANG Pei-Long, ZHANG Wei, XUE Yan, LI Yu. Simultaneous Detection of 21 Kinds of Mycotoxins and Their Metabolites in Animal Plasma with Impurity Adsorption Purification Followed by Liquid Chromatography-Tandem Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(2): 231-237. doi: 10.11895/j.issn.0253-3820.160526 shu

Simultaneous Detection of 21 Kinds of Mycotoxins and Their Metabolites in Animal Plasma with Impurity Adsorption Purification Followed by Liquid Chromatography-Tandem Mass Spectrometry

WANG Rui-Guo ^1, ,
SU Xiao-Ou ¹ ,
WANG Pei-Long ¹ ,
ZHANG Wei ¹ ,
XUE Yan ² ,
LI Yu ²

1.
Institute of Quality Standards and Testing Technology for Agricultural Products, Chinese Academy of Agricultural Science, Key Laboratory of Agrifood Safety and Quality, Ministry of Agriculture, Beijing 100081, China;
2.
Alltech Biological Products(China) Co., LTD, Beijing 100600, China

Received Date: 9 July 2016
Revised Date: 19 September 2016

Fund Project: This work was supported by the Planning Subject of the Twelfth Five-Plan National Science and Technology for the Rural Development in China (No.2011BAD26B0405)

A novel method for simultaneous detection of mycotoxins (e.g., aflatoxin B₁) or their metabolic residues in animal plasma with impurity adsorption purification followed ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed. Extraction of mycotoxins and their metabolites from animal plasma sample was performed with 0.1% formic acid-acetonitrile solution after addition of sodium chloride and hydrous magnesium sulfate. The extract was then dehydrated and purified with hydrous magnesium sulfate, C₁₈, primary secondary amine, and alumina-A. 3 mL of the supernatant was evaporated and re-dissolved with 0.5 mL of 0.1% formic acid aqueous solution/acetonitrile (70:30, V/V) for UPLC-MS/MS detection. The analytes were separated by a C₁₈ column utilizing gradient elution with 0.1% formic acid aqueous solution containing 0.5 mmol of ammonium acetate and 0.1% formic acid-methanol solution, and finally detected by tandem mass spectrometry in positive/negative ESI mode. Identification and quantification were achieved by LC-MS/MS with multi-reaction monitoring (MRM). Good linearity in response was obtained in the analytes concentration range of 0.05-100 ng/mL with correlation coefficients larger than 0.99. The limits of quantification (S/N=10) were around 0.05-0.5 ng/mL. The recoveries of mycotoxins and their metabolites spiked in blank plasma samples were in the range of 62.0%-116.4%, with relative standard deviations (RSDs) less than 19.0%.
- Liquid chromatography-tandem mass spectrometry,
- Plasma,
- Mycotoxins,
- Residue
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