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Citation: ZHOU Guang-Yun, WANG Gui-Ji, REN Hao-Wei, LU Qian, YANG Yan, GUO Li-Hai, LIU Ning. Ultra Performance Liquid Chromatography-Tandem Mass Spectrometric Method for Detection of Pork Peptide Biomarkers[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(2): 205-210. doi: 10.11895/j.issn.0253-3820.160491 shu

Ultra Performance Liquid Chromatography-Tandem Mass Spectrometric Method for Detection of Pork Peptide Biomarkers

ZHOU Guang-Yun ^1,2, ,
WANG Gui-Ji ^1,2 ,
REN Hao-Wei ^1,2 ,
LU Qian ¹ ,
YANG Yan ^1,2 ,
GUO Li-Hai ³ ,
LIU Ning ^1,2

1.
Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University, Harbin 150030, China;
2.
Synergetic Innovation Center of Food Safety and Nutrition, Harbin 150028, China;
3.
Shanghai AB Sciex Analytical Instrument Trading Co., Ltd, Beijing 1000015, China

Received Date: 28 June 2016
Revised Date: 11 November 2016

The samples of muscular tissue from pork, beef and lamb which were closely related in the genetic relationship were analyzed by ultra performance liquid chromatography-tandem mass spectrometric (UPLC-MS) technique. The specific peptide biomarkers of pig meat species were found and confirmed. Proteins from three pure meat samples were extracted and digested using trypsin, the digested proteins were identified by UPLC-triple time-of-flight (TOF)-MS, and the total ion chromatogram (TIC) was searched and analyzed against the UniProt database. Three high abundant homologous proteins of three species and 8 potential peptide biomarkers of pork were found. A multiple reaction monitoring (MRM) QTRAP-MS method was established to confirm the specificity of potential peptide biomarkers. As a result, five peptide biomarkers of pig species meat were confirmed, three of which were not reported.
- Pork,
- Ultra performance liquid chromatography-tandem mass spectrometry,
- Multiple reaction monitoring,
- Peptide biomarkers
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