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Citation: SUN Ming, HONG Wei, SHU Jing, LI Li. Producing Collagen Nano-fibril Arrays by the Molecular Broom Mechanism of Atomic Force Microscopic Tip[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(10): 1471-1476. doi: 10.11895/j.issn.0253-3820.160418 shu

Producing Collagen Nano-fibril Arrays by the Molecular Broom Mechanism of Atomic Force Microscopic Tip

School of Electronic Engineering and Optoelectronic Technology, Nanjing University of Science and Technology, Nanjing 210094, China

Received Date: 1 June 2016
Revised Date: 10 August 2016

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 51303082)

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By taking advantage of atomic force microscopes'(AFM) capability of manipulating and processing materials on the microscale, we discovered and investigated a top-down preparation method of biological macromolecular nano-fibril arrays. 50 μg/mL solution of rat tail type I collagen monomers was used to coat the mica surface to form a protein membrane. In the AFM Contact Mode, the AFM tip then manipulated the membrane with appropriate force between 100 nN and 1 mN, thus producing patterned collagen nano-fibril arrays with specific orientations. The nano-fibrils are averagely 2-5 nm in height and 150-350 nm in width. Based on the relationship between the structure of such nano-fibril arrays and the AFM scanning patterns, we investigated and discussed the formation of the protein fibril arrays, and verified the molecular broom mechanism of the AFM tip. This preparation method could potentially provide an efficient technique to manufacture cell culture vessels, produce biological probes with high specificities, and synthesize novel micro/nano materials as well.
- Collagen,
- Atomic force microscope,
- Molecular broom mechanism,
- Nano-fibril,
- Top-down
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