Citation: ZHANG Ya-Nan, YANG Xing-Lun, BIAN Yong-Rong, GU Cheng-Gang, WANG Dai-Zhang, JIANG Xin. An Accelerated Solvent Extraction-Solid Phase Extraction-High Performance Liquid Chromatographic Method for Determination of Polycyclic Aromatic Hydrocarbons in Soil and Earthworm Samples[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(10): 1514-1520. doi: 10.11895/j.issn.0253-3820.160328 shu

An Accelerated Solvent Extraction-Solid Phase Extraction-High Performance Liquid Chromatographic Method for Determination of Polycyclic Aromatic Hydrocarbons in Soil and Earthworm Samples

1.
Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;
2.
University of Chinese Academy of Sciences, Beijing 100049, China;
3.
College of Resources and Environment, Henan Agricultural University, Zhengzhou 450002, China

Received Date: 25 April 2016
Revised Date: 9 August 2016

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 41271327, 41271464, 21377138) and 135 Forntie Projects of Institute of Soil Science, Chinese Academy of Sciences (Nos. ISSASIP1621, ISSASIP1618)

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A high performance liquid chromatographic (HPLC) method was extablished for determination of the content of 7 polycyclic aromatic hydrocarbons (PAHs) in soil and earthworm samples based on accelerated solvent extraction (ASE) for extraction and solid phase extraction (SPE) column for sample purification. In this method, the samples were first extracted by the mixed solution of n-hexane and acetone extraction (4:1, V/V), and then purified by SPE column (silica gel column for soil sample purification and Al₂O₃-silica gel column for earthworm sample purification), eluted by 10 mL of elution of n-hexane and dichloromethane (9:1, V/V), and evaporated to dryness with rotary evaporator. After that, the pretreated samples were re-dissolved with acetonitrile to a constant volume, followed by a filtration with a 0.22-μm organic filter membrane for the quantification by HPLC. The recoveries of the method for 7 PAHs were 83.5%-110.2% in soil samples and 81.2%-97.1% in earthworm samples. The detection limits of the method for 7 PAHs were 0.15-0.85 μg/kg. The method had good reproducibility and met the quality control requirements of sample analysis.
- Accelerated solvent extraction,
- Solid phase extraction,
- High performance liquid chromatography,
- Soil,
- Earthworm,
- Polycyclic aromatic hydrocarbons
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沈阳化工大学材料科学与工程学院沈阳 110142