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Citation: WU Li-Dong, LIU Ling, LI Dan, FU Xiao-Chen, LIU Huan, SONG Yi, MA Bing, ZHAI Jun-Feng. A New Microbial Biosensor for Detecting and Monitoring Water Acute Toxicity Based on Methylene Blue[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(9): 1354-1358. doi: 10.11895/j.issn.0253-3820.160245 shu

A New Microbial Biosensor for Detecting and Monitoring Water Acute Toxicity Based on Methylene Blue

WU Li-Dong ^1, ,
LIU Ling ² ,
LI Dan ² ,
FU Xiao-Chen ¹ ,
LIU Huan ¹ ,
SONG Yi ¹ ,
MA Bing ¹ ,
ZHAI Jun-Feng ²

1.
Chinese Academy of Fishery Sciences, Beijing 344000, China
2.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China

Received Date: 30 March 2016
Revised Date: 15 June 2016

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 31301177, 21405148), the Ministry of Science and Technology of China (No. 2013YQ170585), and the Foundation of Young Scholars of Jilin Province (No. 20150520010JH)

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A novel method based on methylene blue (MB) was established for the rapid determination of acute water toxicity with the P. fluorescens as the test organism and the damage of cell membrane as the toxicity response. In this method, the damaged rather than intact cells could be stained positively by MB. Therefore, in the presence of toxicants that could induce the damage of cell membrane, MB would enter into cells, resulting in a decrease in MB concentration of the solution. The acute water toxicity could thus be determined by measuring the changes optically in the concentration of MB in the solutions. The present results displayed that, the detection limits of the reported method for 3,5-dichlorophenol, As³⁺ and Hg²⁺ were 1.6, 12.5 and 3.2 mg/L, respectively. In special, the detection limit of the reported method for 3,5-dichlorophenol (DCP) was lower than that of the commercial equipment, Baroxymeter. The reported method is simple, rapid and reliable, and shows an excellent promise in the determination and monitoring of acute toxicity.
- Acute water toxicity,
- Methylene blue,
- Intact cell,
- Damaged cell,
- P. fluorescens
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