Citation: ZENG Yuan, LUO Li-Qiang. Mechanism of Lead Biosorption and Biotransformation in Lead-Resistant Bacteria by In Situ Synchrotron Radiation Micro X-Ray Fluorescence and X-Ray Absorption Near Edge Structure[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(9): 1372-1377. doi: 10.11895/j.issn.0253-3820.160080 shu

Mechanism of Lead Biosorption and Biotransformation in Lead-Resistant Bacteria by In Situ Synchrotron Radiation Micro X-Ray Fluorescence and X-Ray Absorption Near Edge Structure

ZENG Yuan ^1,2, ,
LUO Li-Qiang ^1,2

1.
National Research Center for Geoanalysis, Beijing 100037, China
2.
China University of Geosciences, Beijing 100083, China

Received Date: 29 January 2016
Revised Date: 15 June 2016

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 20775018, 41201527) and the National High Technology Research and Development Program of China (No. 2007AA06Z124)

Figures(4)

To investigate the mechanism of lead biosorption and biotransformation in lead-resistant bacteria in microcosmic scale, synchrotron radiation micro X-ray fluorescence (μ-SRXRF) and X-ray absorption near edge structure (XANES) was used to determine the element distribution characteristics and speciation of lead(Pb) in lead-resistant bacteria strain isolated from farmland soil samples taken from a lead-zinc mine in Lanping county, Yunnan province in China. The isolated species was identified to be Arthrobacter sp. The concentration of Pb biosorpted in lead-resistant bacteria was directly determined by μ-SRXRF, and the results showed that the highest concentration was 5925 μg/g and the bioaccumulate factor was 14.8. Speciation of Pb in lead-resistant bacteria B2, LB (Lysogeny Broth) medium and soil were also presented after using Pb L_Ⅲ XANES. Compared with Pb L_Ⅲ XANES peak shape and peak position between standard samples, it was demonstrated that the Pb in lead-resistant bacteria was mainly formed by amorphous forms like PbS (58.0%), (C₁₇H₃₅COO)₂Pb (22.2%), Pb₅(PO₄)₃Cl (19.8%) rather than organic Pb speciation which was exist in LB medium. Therefore, the isolated lead-resistant bacteria could biotransform Pb to sulfuric compounds. The biotransformation regular could be of great interest for other investigators as reference in study of bioavailability impact factor of heavy metals.
- Micro-synchrotron radiation X-ray fluorescence,
- X-ray absorption near edge structure,
- Lead-zinc mine,
- Lead-resistant bacteria,
- Lead,
- Speciation
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
15. [15]
16. [16]
17. [17]
18. [18]
19. [19]
20. [20]
21. [21]
22. [22]
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