Home

Citation: XU Duo-Qi, ZHANG Lei-Ping, WANG Ji-Fen, HUANG Jian, GUO Zhen, LIN Kuan. Determination of Zaleplon and Its Metabolites in Human Blood by QuEChERS-Ultra Performance Liquid Chromatography-Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(7): 1059-1064. doi: 10.11895/j.issn.0253-3820.160033 shu

Determination of Zaleplon and Its Metabolites in Human Blood by QuEChERS-Ultra Performance Liquid Chromatography-Mass Spectrometry

1.
College of Criminal Science and Technology, People's Public Security University of China, Beijing 100038, China;
2.
Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China

Received Date: 13 January 2016
Revised Date: 14 April 2016

Figures(3)

A fast testing method was established for the determination of zaleplon and its two metabolites, 5-oxozaleplon and desethylzaleplon, in blood by ultra performance liquid chromatography tandem electrospray ionization triple quadrapole-mass spectrometry (UPLC-TQ/MS). The optimized QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method was used for sample preparation. The blood samples were extracted with 0.1% acetic acid and dehydrated by adding anhydrous magnesium sulfate. The targets were separated on a Waters BEH C₁₈ column by gradient elution with 0.1% formic acid-0.1% formic acid/acetonitrile as mobile phase, ionized with positive electrospray (ESI⁺), and detected under multiple reaction monitoring (MRM) mode. As a result, zaleplon and its two metabolites displayed excellent linearity in the concentration of 0.5-100 ng/mL (R²>0.997). The average recoveries ranged from 92.0% to 100.1%. The RSD were in the range of 1.9%-5.3%. Besides, the limit of detection(LOD)of this method for target drugs was 0.05 ng/mL (S/N=3), while limits of quantification (LOQ) were in the range of 0.1-0.5 ng/mL (S/N=10). This method can meet the demand of rapidity and accuracy in the analysis of actual cases.
- Zaleplon,
- 5-Oxozaleplon,
- Desethylzaleplon,
- QuEChERS,
- Ultra performance liquid chromatography-mass spectrometry
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
15. [15]
16. [16]
17. [17]
18. [18]
19. [19]
20. [20]
21. [21]
22. [22]
23. [23]
24. [24]
25. [25]
26. [26]
1. [1]
  Yingxian Wang , Tianye Su , Limiao Shen , Jinping Gao , Qinghe Wu . Introduction of Chinese Lacquer from the Perspective of Chemistry: Popularizing Chemistry in Lacquer and Inherit Lacquer Art. University Chemistry, 2024, 39(5): 371-379. doi: 10.3866/PKU.DXHX202312015
2. [2]
  Liqiang Hao , Boyi Nie , Ziping Wan , Jianghua Qiu . The Role of SOD in Skincare: A Chemical Science Popularization Experiment. University Chemistry, 2025, 40(7): 241-248. doi: 10.12461/PKU.DXHX202409084
3. [3]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
4. [4]
  Lubing Qin , Fang Sun , Meiyin Li , Hao Fan , Likai Wang , Qing Tang , Chundong Wang , Zhenghua Tang . Atomically Precise (AgPd)₂₇ Nanoclusters for Nitrate Electroreduction to NH₃: Modulating the Metal Core by a Ligand Induced Strategy. Acta Physico-Chimica Sinica, 2025, 41(1): 100008-0. doi: 10.3866/PKU.WHXB202403008
5. [5]
  Jiying Liu , Zehua Li , Wenjing Zhang , Donghui Wei . Molecular Orbital and Nucleus-Independent Chemical Shift Calculations for C₆H₆ and B₁₂H₁₂^2-: A Computational Chemistry Experiment. University Chemistry, 2025, 40(3): 186-192. doi: 10.12461/PKU.DXHX202406085
6. [6]
  Shunü Peng , Huamin Li , Zhaobin Chen , Yiru Wang . Simultaneous Application of Multiple Quantitative Analysis Methods in Gas Chromatography for the Determination of Active Ingredients in Traditional Chinese Medicine Preparations. University Chemistry, 2025, 40(10): 243-249. doi: 10.12461/PKU.DXHX202412043
7. [7]
  Yifan Xie , Liyun Yao , Ruolin Yang , Yuxing Cai , Yujie Jin , Ning Li . Exploration and Practice of Online and Offline Hybrid Teaching Mode in High-Performance Liquid Chromatography Experiment. University Chemistry, 2025, 40(11): 100-107. doi: 10.12461/PKU.DXHX202412133
8. [8]
  Jingzhao Cheng , Shiyu Gao , Bei Cheng , Kai Yang , Wang Wang , Shaowen Cao . Construction of 4-Amino-1H-imidazole-5-carbonitrile Modified Carbon Nitride-Based Donor-Acceptor Photocatalyst for Efficient Photocatalytic Hydrogen Evolution. Acta Physico-Chimica Sinica, 2024, 40(11): 2406026-0. doi: 10.3866/PKU.WHXB202406026
9. [9]
  Runjie Li , Hang Liu , Xisheng Wang , Wanqun Zhang , Wanqun Hu , Kaiping Yang , Qiang Zhou , Si Liu , Pingping Zhu , Wei Shao . 氨基酸的衍生及手性气相色谱分离创新实验. University Chemistry, 2025, 40(6): 286-295. doi: 10.12461/PKU.DXHX202407059
10. [10]
  Wen Shi , Jiuxing Jiang . 化学中的数学方法课程建设探索. University Chemistry, 2025, 40(6): 48-53. doi: 10.12461/PKU.DXHX202408088
11. [11]
  Zhentong Zhu , Peiyao Du , Chaoqin Zeng , Rui Zhou , Xiaoyan He , Bingzhang Lu , Xiaoquan Lu . Discussion on Teaching Methods for Bilingual Courses in Instrumental Analysis for Chemistry Majors. University Chemistry, 2025, 40(10): 39-45. doi: 10.12461/PKU.DXHX202411014
12. [12]
  Hao Chen , Dongyue Yang , Gang Huang , Xinbo Zhang . Progress on Liquid Organic Electrolytes of Li-O₂ Batteries. Acta Physico-Chimica Sinica, 2024, 40(7): 2305059-0. doi: 10.3866/PKU.WHXB202305059
13. [13]
  Hongwei Ma , Hui Li . Three Methods for Structure Determination from Powder Diffraction Data. University Chemistry, 2024, 39(3): 94-102. doi: 10.3866/PKU.DXHX202310035
14. [14]
  Feng Liang , Desheng Li , Yuting Jiang , Jiaxin Dong , Dongcheng Liu , Xingcan Shen . Method Exploration and Instrument Innovation for the Experiment of Colloid ζ Potential Measurement by Electrophoresis. University Chemistry, 2024, 39(5): 345-353. doi: 10.3866/PKU.DXHX202312009
15. [15]
  Yuting Zhang , Zhiqian Wang . Methods and Case Studies for In-Depth Learning of the Aldol Reaction Based on Its Reversible Nature. University Chemistry, 2024, 39(7): 377-380. doi: 10.3866/PKU.DXHX202311037
16. [16]
  Sifang Zhang , Yanli Tan , Yu Tao , Jiaoyan Zhao , Haihong Zhu . Exploration and Practice of Ideological and Political Cases in the Course of Chemistry History and Methodology. University Chemistry, 2024, 39(10): 377-388. doi: 10.12461/PKU.DXHX202312067
17. [17]
  Yuyang Xu , Ruying Yang , Yanzhe Zhang , Yandong Liu , Keyi Li , Zehui Wei . Research Progress of Aflatoxins Removal by Modern Optical Methods. University Chemistry, 2024, 39(11): 174-181. doi: 10.12461/PKU.DXHX202402064
18. [18]
  Hongting Yan , Aili Feng , Rongxiu Zhu , Lei Liu , Dongju Zhang . Reexamination of the Iodine-Catalyzed Chlorination Reaction of Chlorobenzene Using Computational Chemistry Methods. University Chemistry, 2025, 40(3): 16-22. doi: 10.12461/PKU.DXHX202403010
19. [19]
  Xingyuan Lu , Yutao Yao , Junjing Gu , Peifeng Su . Energy Decomposition Analysis and Its Application in the Many-Body Effect of Water Clusters. University Chemistry, 2025, 40(3): 100-107. doi: 10.12461/PKU.DXHX202405074
20. [20]
  Yu'ang Liu , Yuechao Wu , Junyu Huang , Tao Wang , Xiaohong Liu , Tianying Yan . Computation of Absolute Electrode Potential of Standard Hydrogen Electrode Using Ab Initio Method. University Chemistry, 2025, 40(3): 215-222. doi: 10.12461/PKU.DXHX202407112

Get Citation

PDF

XML

Metrics

PDF Downloads(3)
Abstract views(1254)
HTML views(150)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Address：Zhongguancun North First Street 2,100190 Beijing, PR China Tel: +86-010-82449177-888

DownLoad: Full-Size Img PowerPoint

Return