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Citation: YANG Ting-Ting, LÜ Yi, ZHANG Dong-Sheng, TANG Ye, YUAN Yi, ZHANG Hai-Tao, WANG Hai-Feng. Preparation and Application of Immunoaffinity Column for Multi-mycotoxins[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(8): 1243-1249. doi: 10.11895/j.issn.0253-3820.150999 shu

Preparation and Application of Immunoaffinity Column for Multi-mycotoxins

1.
Jiangsu Suwei Institute of Microbiology Co., LTD, Wuxi 214063, China;
2.
Grain and Oil Quality Monitoring Bureau of Grain in Jiangsu Province, Nanjing 210011, China;
3.
Nanjing university of finance and economics, Nanjing 210011, China

Received Date: 18 December 2015
Revised Date: 5 May 2016

Figures(2)

The three-in-one immunoaffinity column (IAC) for the determination of aflatoxin B₁ (AFB₁)-zearalenone (ZEN)-deoxynivalenol (DON) was prepared with rProtein A-sepharose 4B as the column matrix. The comprehensive performance (such as nonspecific adsorption, column blank, column capacity, column efficiency and sample standard addition recovery rate) was evaluated and investigated. The results showed that, the column capacities of AFB₁, ZEN, DON were 295 ng per 0.25 mL gel, 905 ng per 0.25 mL gel, 2342 ng per 0.25 mL gel, respectively, and the column blank was 0. The average recoveries of AFB₁, ZEN and DON were 97.4%, 98.0% and 98.4%, respectively. By optimizing conditions, the samples were extracted using the mixture of methanol and water (80:20, V/V), and diluted with phosphate buffered saline (contain 0.1% Tween-20, PBST). The detection results of FAPAS (Food Analysis Performance Assessment Scheme) by different batch three-in-one columns were close to the target value. The prepared three-in-one immunoaffinity column which could take the place of conventional single immunoaffinity column was able to meet the requirement for treatment of food and feed samples, and lay a foundation for one step enrichment, purification and detection of multi-mycotoxins.
- Aflatoxin B₁,
- Zearalenone,
- Deoxynivalenol,
- Directed coupling,
- Immunoaffinity column
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