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Citation: HONG Xiao-Yu, LI Shui-Ming, WANG Yong. Analysis of Urine Peptidomic and Post-translation Modifications by Nano Liquid Chromatography-High Resolution Tandem Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(5): 716-722. doi: 10.11895/j.issn.0253-3820.150809 shu

Analysis of Urine Peptidomic and Post-translation Modifications by Nano Liquid Chromatography-High Resolution Tandem Mass Spectrometry

1.
College of Life and Ocean Science, Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen 518060, China;2 Shenzhen Key Laboratory of Marine Bioresources and Ecology, Shenzhen University, Shenzhen 518060, China

Received Date: 14 October 2015
Revised Date: 5 January 2016

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 31470804)

Peptidomics is an emerging field branching from proteomincs that targets endogenous peptides of a whole organism or a subsystem. Many peptides in body fluids including urine are biomarkers with higher clinical sensitivity and specificity. In this study, we separated and enriched these peptides in human urine by the graphene oxide-lanthanum phosphate composite nanomaterial (LaGM), then identified and analyzed by nano liquid chromatography-high resolution tandem mass spectrometry. In single urine sample, 790 peptides which belong to 123 proteins were identified, and some post-translational modifications including oxidation, phosphorylation, deamidated etc, were also identified. There existed a series of peptide ladders in urine peptidomic. At last, the string analysis on protein level revealed strong interaction among the proteins which the identified peptides belonged. This method can provide support for finding the biomarkers of disease in urine.
- Peptidomic,
- Nano liquid chromatography-high resolution tandem mass spectrometry,
- Urine,
- Post-translational modifications
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