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Citation: LIU Yong-Li, WANG Chang-Shun, WANG Xiao-Lei, YANG Shu-Nan, LIU Xing-Guo, FENG Li. Simultaneous Quantification of Morroniside, Loganin, Peoniflorin, Paeonol in Mingmudihuang Pill by Online Two-dimensional Column Switching Ultra Performance Liquid Chromatography[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(5): 828-832. doi: 10.11895/j.issn.0253-3820.150780 shu

Simultaneous Quantification of Morroniside, Loganin, Peoniflorin, Paeonol in Mingmudihuang Pill by Online Two-dimensional Column Switching Ultra Performance Liquid Chromatography

1.
Hebei Institute for Drug Control, Shijiazhuang 050011, China;
2.
Thermofisher Scientific(China) Corporation, Beijing 10080, China

Received Date: 8 October 2015
Revised Date: 15 December 2015

An online Two-dimensional column switching ultra performance liquid chromatography (2D-UPLC) method was established for simultaneous quantification of morroniside, loganin, peoniflorin, paeonol in Mingmudihuang Pill. The first-dimensional column was Thermo Accucore XL C₁₈ (250 mm×2.1 mm, 4 μm) while the second-dimensional column was DIONEX Acclaim phenyl-1 (150 mm×4.6 mm, 3 μm). Acetonitrile and water were used as mobile phase with a gradient elution in the first-dimensional analysis and the second-dimensional analysis used acetonitrile-water as mobile phase with a isocratic elution. The valve switching time was 14.5 min, the detection wavelength was set at 240 nm in 0-14.5 min and 275 nm in 14.5-30 min. The flow rate was 0.5 mL/min, and the column temperature was 30℃. The determination of four compounds in Mingmudihuang Pill was completed in 30 min and it could effectually segregate the isomer of morroniside. The linear ranges of morroniside, loganin, peoniflorin and paeonol were 7.6-377 mg/L, 9.2-459 mg/L, 8.4-419 mg/L and 8.2-409 mg/L, respectively, and all the correlation coefficients (r) were>0.9999. The recoveries of the four compounds were 98.3%-100.2%. It was proved that this method could greatly improve the sufficiency of sample analysis and could control the quality of Mingmudihaung Pill efficiently.
- Online two-dimensional column switching ultra performance liquid chromatography,
- Mingmudihuang pill,
- Morroniside,
- Loganin,
- Peoniflorin,
- Paeonol,
- Isomer
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