Citation: FAN Xue-Mei, WANG Shu-Min, LI Zhe-Jian, ZHENG Xing-Wang. Determination of Uric Acid Based on Chitosan/Ru(bpy)₃²⁺/Silica Nanoparticles Electrochemiluminescence Sensor[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(3): 342-347. doi: 10.11895/j.issn.0253-3820.150705 shu

Determination of Uric Acid Based on Chitosan/Ru(bpy)₃²⁺/Silica Nanoparticles Electrochemiluminescence Sensor

1.
¹ College of Chemical Engineering and Modern Materials, Shangluo University, Shangluo 726000, China;
2.
² School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China

Corresponding author: FAN Xue-Mei,
Received Date: 6 September 2015
Available Online: 29 December 2015
Fund Project: 本文系国家自然科学基金(No.30970696) (No.30970696)陕西省科技统筹创新工程计划项目(2012KTDZ02-02) (2012KTDZ02-02)商洛市科技局计划项目(SK-2014-4) (SK-2014-4)陕西省尾矿资源综合利用重点实验室项目(14SKY-FWK06)资助。 (14SKY-FWK06)

Chitosan-Ru(bpy)₃²⁺-SiO₂ composite nanoparticles (CRuS NPs) were prepared by reverse microemulsion method, and based on the Nafion/MCNT composite membrane technology, CRuS NPs were effectively and steadily immobilize on the surface of a glassy carbon electrode to prepare the electrochemiluminescence sensor for uric acid determination. In 0.1 mol/L PBS (pH 7.4) buffer solution, when the actuation duration between uric acid and the modified electrode was 15 min, the electrochemiluminescence showed a good linear relationship to the negative logarithm of uric acid concentration in the range of 1.0 × 10^-10-1.0 × 10^-5 mol/L, the linear equation was I_ECL=-709.52-202.74lgC and the correlation coefficient was 0.9936 with a detection limit of 6.0 × 10^-12 mol/L. The ECL sensor exhibited excellent repeatability and stability, and the RSD for 11 times determination of 1.0 × 10^-8 mol/L uric acid was 2.9%. The recovery was 98.5%-103.5% in the determination of real Uric acid sample.
- Electrogenerated chemiluminescence,
- Chitosan-Ru(bpy)₃²⁺-SiO₂ nanoparticles,
- Uric acid
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Determination of Uric Acid Based on Chitosan/Ru(bpy)32+/Silica Nanoparticles Electrochemiluminescence Sensor

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通讯作者: 陈斌, bchen63@163.com

Determination of Uric Acid Based on Chitosan/Ru(bpy)₃²⁺/Silica Nanoparticles Electrochemiluminescence Sensor